Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12186 | 5' | -59.9 | NC_003309.1 | + | 10718 | 0.67 | 0.453945 |
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Target: 5'- gCG-CGAGCgaagccUCGGCGCgCUCGAGCu-- -3' miRNA: 3'- -GCaGCUCG------AGCUGCGgGAGCUCGccc -5' |
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| 12186 | 5' | -59.9 | NC_003309.1 | + | 25868 | 0.69 | 0.34918 |
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Target: 5'- aCGUCgGGGCgcCGAacaGCaCCggcgCGAGCGGGa -3' miRNA: 3'- -GCAG-CUCGa-GCUg--CG-GGa---GCUCGCCC- -5' |
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| 12186 | 5' | -59.9 | NC_003309.1 | + | 1707 | 0.69 | 0.341264 |
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Target: 5'- -aUCGAGCUUGcGCGCCaugUCGAGCGcGa -3' miRNA: 3'- gcAGCUCGAGC-UGCGGg--AGCUCGC-Cc -5' |
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| 12186 | 5' | -59.9 | NC_003309.1 | + | 45764 | 0.72 | 0.213834 |
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Target: 5'- -uUCGGGUUCGGCGCCUgauguacgcgccggUCGuacAGCGGGu -3' miRNA: 3'- gcAGCUCGAGCUGCGGG--------------AGC---UCGCCC- -5' |
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| 12186 | 5' | -59.9 | NC_003309.1 | + | 49655 | 1.09 | 0.000467 |
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Target: 5'- uCGUCGAGCUCGACGCCCUCGAGCGGGc -3' miRNA: 3'- -GCAGCUCGAGCUGCGGGAGCUCGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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