miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12187 3' -60.8 NC_003309.1 + 28483 0.66 0.432898
Target:  5'- cGCGcGCCacGCCGGcccguauagCGGcgaUUGCCCGAGg -3'
miRNA:   3'- -CGC-CGGcuCGGCCa--------GUC---AACGGGCUC- -5'
12187 3' -60.8 NC_003309.1 + 19444 0.67 0.37105
Target:  5'- cGCGGCgGAGCCG-UCA----CCCGGGa -3'
miRNA:   3'- -CGCCGgCUCGGCcAGUcaacGGGCUC- -5'
12187 3' -60.8 NC_003309.1 + 35827 0.67 0.357784
Target:  5'- cGCauUCGAGCUGGUCGGcgcaaucgacuuacUGCCCGAGu -3'
miRNA:   3'- -CGccGGCUCGGCCAGUCa-------------ACGGGCUC- -5'
12187 3' -60.8 NC_003309.1 + 49940 1.09 0.000319
Target:  5'- gGCGGCCGAGCCGGUCAGUUGCCCGAGc -3'
miRNA:   3'- -CGCCGGCUCGGCCAGUCAACGGGCUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.