Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12189 | 3' | -56.5 | NC_003309.1 | + | 22626 | 0.66 | 0.658212 |
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Target: 5'- -cGCCgGGCAcGC-CGUCGccaccgGCUGCCg -3' miRNA: 3'- uuUGGgCCGU-CGcGCAGCca----UGAUGG- -5' |
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| 12189 | 3' | -56.5 | NC_003309.1 | + | 43783 | 0.66 | 0.658212 |
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Target: 5'- -cGCCCGGCAaacgucucuccGCGCaUCGGcguCUGuCCg -3' miRNA: 3'- uuUGGGCCGU-----------CGCGcAGCCau-GAU-GG- -5' |
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| 12189 | 3' | -56.5 | NC_003309.1 | + | 17533 | 0.66 | 0.647259 |
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Target: 5'- uGGAUCUGGCgcacccacGGCGCGUCGG-ACgucagcggcacGCCg -3' miRNA: 3'- -UUUGGGCCG--------UCGCGCAGCCaUGa----------UGG- -5' |
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| 12189 | 3' | -56.5 | NC_003309.1 | + | 15638 | 0.66 | 0.604499 |
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Target: 5'- aGGACCgCGGUAGUGCGaUUGGcagaucgaaauaauCUGCCg -3' miRNA: 3'- -UUUGG-GCCGUCGCGC-AGCCau------------GAUGG- -5' |
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| 12189 | 3' | -56.5 | NC_003309.1 | + | 15539 | 0.67 | 0.549193 |
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Target: 5'- cAAACCCGGCuGCuCGUCGcucuuCUGCUc -3' miRNA: 3'- -UUUGGGCCGuCGcGCAGCcau--GAUGG- -5' |
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| 12189 | 3' | -56.5 | NC_003309.1 | + | 7818 | 0.68 | 0.517381 |
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Target: 5'- cAGCCCGGCgAGCGCcauGUCGaGcGC-GCCa -3' miRNA: 3'- uUUGGGCCG-UCGCG---CAGC-CaUGaUGG- -5' |
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| 12189 | 3' | -56.5 | NC_003309.1 | + | 11245 | 0.69 | 0.44637 |
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Target: 5'- cAGACuCCGGUAcgcggacuGCGCGUCGu--CUGCCg -3' miRNA: 3'- -UUUG-GGCCGU--------CGCGCAGCcauGAUGG- -5' |
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| 12189 | 3' | -56.5 | NC_003309.1 | + | 47973 | 0.69 | 0.44637 |
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Target: 5'- -uGCCCGuGCGGCGCGccagcucgcgcUCGGcgaaGCUGCg -3' miRNA: 3'- uuUGGGC-CGUCGCGC-----------AGCCa---UGAUGg -5' |
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| 12189 | 3' | -56.5 | NC_003309.1 | + | 52322 | 1.1 | 0.000584 |
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Target: 5'- gAAACCCGGCAGCGCGUCGGUACUACCg -3' miRNA: 3'- -UUUGGGCCGUCGCGCAGCCAUGAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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