Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12193 | 3' | -52.6 | NC_003309.1 | + | 32850 | 0.66 | 0.856473 |
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Target: 5'- aGGCG-UGCUUC-GCGa---GCUGCGGg -3' miRNA: 3'- -CCGUaAUGAAGaCGCaacgCGGCGCC- -5' |
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| 12193 | 3' | -52.6 | NC_003309.1 | + | 9558 | 0.66 | 0.842624 |
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Target: 5'- cGGCG--GCUUgUGCGUauUcgcggaucgaacugcGCGCCGCGu -3' miRNA: 3'- -CCGUaaUGAAgACGCA--A---------------CGCGGCGCc -5' |
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| 12193 | 3' | -52.6 | NC_003309.1 | + | 12055 | 0.66 | 0.830026 |
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Target: 5'- cGGCGUUGgUggauacGCGUgacgucUGCGCCGCa- -3' miRNA: 3'- -CCGUAAUgAaga---CGCA------ACGCGGCGcc -5' |
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| 12193 | 3' | -52.6 | NC_003309.1 | + | 42288 | 0.67 | 0.823568 |
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Target: 5'- cGGCcgUACUUCUucucgagGUGUuuccacagagcgauuUcgaGUGCCGCGGg -3' miRNA: 3'- -CCGuaAUGAAGA-------CGCA---------------A---CGCGGCGCC- -5' |
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| 12193 | 3' | -52.6 | NC_003309.1 | + | 46755 | 0.68 | 0.750856 |
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Target: 5'- gGGCGUcACUUCgccaGCGc-GCGCCGgaaCGGg -3' miRNA: 3'- -CCGUAaUGAAGa---CGCaaCGCGGC---GCC- -5' |
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| 12193 | 3' | -52.6 | NC_003309.1 | + | 39282 | 0.69 | 0.685926 |
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Target: 5'- uGGCAacucGCgcaUGCGUUGCGUCGCc- -3' miRNA: 3'- -CCGUaa--UGaagACGCAACGCGGCGcc -5' |
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| 12193 | 3' | -52.6 | NC_003309.1 | + | 44289 | 0.72 | 0.503107 |
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Target: 5'- cGUuUUGCUUCUGCcgcuuugcacgaGCGCCGCGGu -3' miRNA: 3'- cCGuAAUGAAGACGcaa---------CGCGGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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