Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12197 | 3' | -47.4 | NC_003309.1 | + | 923 | 0.66 | 0.989707 |
Target: 5'- -aGCUUUcUCGAAacCGGCgCUUGCCGg -3' miRNA: 3'- caUGAAGuAGCUUauGUUG-GAGCGGC- -5' |
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12197 | 3' | -47.4 | NC_003309.1 | + | 22124 | 0.66 | 0.98637 |
Target: 5'- -cGCccgCAUUGA--ACcGCCUCGCCGu -3' miRNA: 3'- caUGaa-GUAGCUuaUGuUGGAGCGGC- -5' |
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12197 | 3' | -47.4 | NC_003309.1 | + | 46227 | 0.68 | 0.960457 |
Target: 5'- uUGC-UCGUCGGGcgACAGCUUCGCa- -3' miRNA: 3'- cAUGaAGUAGCUUa-UGUUGGAGCGgc -5' |
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12197 | 3' | -47.4 | NC_003309.1 | + | 22502 | 0.69 | 0.94193 |
Target: 5'- cGUGgUUCguugGUCGAcgGCGGCCacCGCCGa -3' miRNA: 3'- -CAUgAAG----UAGCUuaUGUUGGa-GCGGC- -5' |
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12197 | 3' | -47.4 | NC_003309.1 | + | 10526 | 0.7 | 0.918532 |
Target: 5'- uGUACUUgAUCGAGc-CGGCCgucaaCGCCGa -3' miRNA: 3'- -CAUGAAgUAGCUUauGUUGGa----GCGGC- -5' |
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12197 | 3' | -47.4 | NC_003309.1 | + | 7633 | 0.7 | 0.904981 |
Target: 5'- gGUGCga-AUCGcccccuCGGCCUCGCCGa -3' miRNA: 3'- -CAUGaagUAGCuuau--GUUGGAGCGGC- -5' |
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12197 | 3' | -47.4 | NC_003309.1 | + | 6255 | 0.73 | 0.789763 |
Target: 5'- aUGCUcgUCGUCGAAcccUGCGAUgUCGCCu -3' miRNA: 3'- cAUGA--AGUAGCUU---AUGUUGgAGCGGc -5' |
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12197 | 3' | -47.4 | NC_003309.1 | + | 47544 | 0.73 | 0.768651 |
Target: 5'- -cGCUUCGUCG-AUGCAGUC-CGCCGg -3' miRNA: 3'- caUGAAGUAGCuUAUGUUGGaGCGGC- -5' |
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12197 | 3' | -47.4 | NC_003309.1 | + | 10780 | 0.74 | 0.713353 |
Target: 5'- -cGCUUUGUCGAucGUGCcuuGCUUCGCCGc -3' miRNA: 3'- caUGAAGUAGCU--UAUGu--UGGAGCGGC- -5' |
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12197 | 3' | -47.4 | NC_003309.1 | + | 2754 | 0.75 | 0.678989 |
Target: 5'- cGUACUUCAgaaUCGAAUACAGcgggugguCCgUUGCCGg -3' miRNA: 3'- -CAUGAAGU---AGCUUAUGUU--------GG-AGCGGC- -5' |
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12197 | 3' | -47.4 | NC_003309.1 | + | 7523 | 0.75 | 0.667417 |
Target: 5'- cGUugUUCggCGAAU-CAGCCUUGCCc -3' miRNA: 3'- -CAugAAGuaGCUUAuGUUGGAGCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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