Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12197 | 5' | -54.7 | NC_003309.1 | + | 44853 | 0.65 | 0.798057 |
Target: 5'- gGCGGCGAUCcgcgcaaGGuUCGcUCGCuuUGuCGCa -3' miRNA: 3'- gCGCCGCUAG-------UC-AGC-AGCGcuAU-GCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 18751 | 0.66 | 0.765599 |
Target: 5'- gGCGGCGAU-AGUCGgcgccuugucgagCGUGAU-CGUc -3' miRNA: 3'- gCGCCGCUAgUCAGCa------------GCGCUAuGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 19179 | 0.66 | 0.759529 |
Target: 5'- -uCGGCGAcgUAGcUCGUCGCGugguuCGCc -3' miRNA: 3'- gcGCCGCUa-GUC-AGCAGCGCuau--GCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 27934 | 0.66 | 0.756476 |
Target: 5'- uCGCGGCGggCAcgcgcaauugauccGUCGUUGCucGAguaugggGCGCc -3' miRNA: 3'- -GCGCCGCuaGU--------------CAGCAGCG--CUa------UGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 1250 | 0.66 | 0.74931 |
Target: 5'- gCGCGGCGcu--GUCGugcUCGUGAUACu- -3' miRNA: 3'- -GCGCCGCuaguCAGC---AGCGCUAUGcg -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 40485 | 0.66 | 0.748282 |
Target: 5'- cCGCGGCGAgggagcCAGaggacgccggaguUgGUCGCGA--CGCa -3' miRNA: 3'- -GCGCCGCUa-----GUC-------------AgCAGCGCUauGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 1049 | 0.67 | 0.728534 |
Target: 5'- aCGCcuGCuuuUCGGUCGUCGCGcc-CGCa -3' miRNA: 3'- -GCGc-CGcu-AGUCAGCAGCGCuauGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 6073 | 0.67 | 0.728534 |
Target: 5'- aCGCGGgcCGA--AGUCGUucuuUGCGAUcACGCg -3' miRNA: 3'- -GCGCC--GCUagUCAGCA----GCGCUA-UGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 5606 | 0.67 | 0.727484 |
Target: 5'- uCGgGGUGuUCGccgugccGUCGUCGCGAaugaACGCc -3' miRNA: 3'- -GCgCCGCuAGU-------CAGCAGCGCUa---UGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 44181 | 0.67 | 0.717999 |
Target: 5'- cCGCGGCGcgCuGcCGcUCGgCG-UACGCg -3' miRNA: 3'- -GCGCCGCuaGuCaGC-AGC-GCuAUGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 17517 | 0.67 | 0.70738 |
Target: 5'- -aCGGCGcGUCGGaCGUCaGCGGcACGCc -3' miRNA: 3'- gcGCCGC-UAGUCaGCAG-CGCUaUGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 19915 | 0.67 | 0.696688 |
Target: 5'- cCGCGGCGcUCAGcagguucgagcUCGaUCGCuGcUGCGCc -3' miRNA: 3'- -GCGCCGCuAGUC-----------AGC-AGCG-CuAUGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 5061 | 0.68 | 0.685936 |
Target: 5'- gCGCGGCGGcCGGU-GcCGCGAcgGCGg -3' miRNA: 3'- -GCGCCGCUaGUCAgCaGCGCUa-UGCg -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 19519 | 0.68 | 0.6708 |
Target: 5'- aCGCGaaauccgagaacuUGAUCGcGUCGUCGCGAUucgGCGUg -3' miRNA: 3'- -GCGCc------------GCUAGU-CAGCAGCGCUA---UGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 10668 | 0.68 | 0.66429 |
Target: 5'- uCGCGGgcgaUGAUCGccgCGUacggcaugccCGCGAUGCGCg -3' miRNA: 3'- -GCGCC----GCUAGUca-GCA----------GCGCUAUGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 45866 | 0.69 | 0.609834 |
Target: 5'- aCGCGGCGA-CGGcCGUCGaucacgaGGUucuCGCc -3' miRNA: 3'- -GCGCCGCUaGUCaGCAGCg------CUAu--GCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 9397 | 0.7 | 0.534599 |
Target: 5'- uGCGGCGGucguauUCGGUCGuauUCGCcGUaACGCu -3' miRNA: 3'- gCGCCGCU------AGUCAGC---AGCGcUA-UGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 16024 | 0.7 | 0.534599 |
Target: 5'- cCGCGGCGGcgUAGUCaUCG-GAUGCGa -3' miRNA: 3'- -GCGCCGCUa-GUCAGcAGCgCUAUGCg -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 41376 | 0.71 | 0.472845 |
Target: 5'- aGCGGCGGgcaAGcCG-CGUGGUugGCu -3' miRNA: 3'- gCGCCGCUag-UCaGCaGCGCUAugCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 47940 | 0.74 | 0.320816 |
Target: 5'- aGCuGCGAccgauaguUCGGUCGUCGCGc-ACGCg -3' miRNA: 3'- gCGcCGCU--------AGUCAGCAGCGCuaUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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