Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12197 | 5' | -54.7 | NC_003309.1 | + | 28461 | 0.74 | 0.320816 |
Target: 5'- aGCGGCGAUUGcccgagguuGUCGUCGCGcAcguucaugugcUACGCa -3' miRNA: 3'- gCGCCGCUAGU---------CAGCAGCGC-U-----------AUGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 17815 | 0.75 | 0.2908 |
Target: 5'- gCGuCGGCGAUC-GUCGcCGUG-UGCGCg -3' miRNA: 3'- -GC-GCCGCUAGuCAGCaGCGCuAUGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 9709 | 0.76 | 0.237451 |
Target: 5'- aGCGGUugccGUguGUCGcgCGCGAUGCGCa -3' miRNA: 3'- gCGCCGc---UAguCAGCa-GCGCUAUGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 22455 | 0.79 | 0.163918 |
Target: 5'- gCGCGGCGAUCGcacgcugccggcGUCGagCGCGGcGCGCg -3' miRNA: 3'- -GCGCCGCUAGU------------CAGCa-GCGCUaUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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