Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12197 | 5' | -54.7 | NC_003309.1 | + | 5606 | 0.67 | 0.727484 |
Target: 5'- uCGgGGUGuUCGccgugccGUCGUCGCGAaugaACGCc -3' miRNA: 3'- -GCgCCGCuAGU-------CAGCAGCGCUa---UGCG- -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 5061 | 0.68 | 0.685936 |
Target: 5'- gCGCGGCGGcCGGU-GcCGCGAcgGCGg -3' miRNA: 3'- -GCGCCGCUaGUCAgCaGCGCUa-UGCg -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 1250 | 0.66 | 0.74931 |
Target: 5'- gCGCGGCGcu--GUCGugcUCGUGAUACu- -3' miRNA: 3'- -GCGCCGCuaguCAGC---AGCGCUAUGcg -5' |
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12197 | 5' | -54.7 | NC_003309.1 | + | 1049 | 0.67 | 0.728534 |
Target: 5'- aCGCcuGCuuuUCGGUCGUCGCGcc-CGCa -3' miRNA: 3'- -GCGc-CGcu-AGUCAGCAGCGCuauGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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