Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12198 | 5' | -48.3 | NC_003309.1 | + | 43257 | 0.67 | 0.963444 |
Target: 5'- cGGCGCUCGGCaacuucacGAGCG-CGU-AACCa -3' miRNA: 3'- -CCGCGAGUUGga------CUUGUaGUAgUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 17792 | 0.67 | 0.963444 |
Target: 5'- cGCGUUCGGCgUGAuACGgcgCAcacggaUCAGCCa -3' miRNA: 3'- cCGCGAGUUGgACU-UGUa--GU------AGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 34871 | 0.67 | 0.955293 |
Target: 5'- aGCGCcgcCAACgUGAacgagcACGUCAUC-ACCg -3' miRNA: 3'- cCGCGa--GUUGgACU------UGUAGUAGuUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 19912 | 0.67 | 0.953972 |
Target: 5'- cGGCGCUCAGCagguucGAGCucgaucgcugcugcgCcgCGACCg -3' miRNA: 3'- -CCGCGAGUUGga----CUUGua-------------GuaGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 42547 | 0.73 | 0.736032 |
Target: 5'- uGGCGCUCAGuCCgcaaguUGAGCGccggccgaAUCAGCCa -3' miRNA: 3'- -CCGCGAGUU-GG------ACUUGUag------UAGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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