Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12198 | 5' | -48.3 | NC_003309.1 | + | 3271 | 0.67 | 0.950788 |
Target: 5'- -aCGUUCGucaucgauagcgGCCUGAACGUCAU--GCCc -3' miRNA: 3'- ccGCGAGU------------UGGACUUGUAGUAguUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 3391 | 0.69 | 0.915535 |
Target: 5'- uGGUGCUCGAUguugCUGAACGuggcucgcuccaacUCGUUcACCa -3' miRNA: 3'- -CCGCGAGUUG----GACUUGU--------------AGUAGuUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 5040 | 0.68 | 0.94599 |
Target: 5'- cGGCGgUCGgauucgggucaACCgGAACAgccgCcgCAGCCg -3' miRNA: 3'- -CCGCgAGU-----------UGGaCUUGUa---GuaGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 6531 | 0.69 | 0.917487 |
Target: 5'- cGGCGC-CAGCCUu-GC-UC-UCGGCCg -3' miRNA: 3'- -CCGCGaGUUGGAcuUGuAGuAGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 10700 | 0.66 | 0.981581 |
Target: 5'- cGCGCUCGAgcuCCUGcuGCG-CggCGGCCg -3' miRNA: 3'- cCGCGAGUU---GGACu-UGUaGuaGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 12621 | 0.7 | 0.856432 |
Target: 5'- cGCGCgCGACCUGcuCcgCcUCGGCCg -3' miRNA: 3'- cCGCGaGUUGGACuuGuaGuAGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 16426 | 0.7 | 0.873392 |
Target: 5'- cGuCGC-CGACCUGcAACGUCAcgucgCGGCCg -3' miRNA: 3'- cC-GCGaGUUGGAC-UUGUAGUa----GUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 17721 | 0.66 | 0.981581 |
Target: 5'- aGCGCUCGuagagcGACGUCGUCuuGCCg -3' miRNA: 3'- cCGCGAGUuggac-UUGUAGUAGu-UGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 17792 | 0.67 | 0.963444 |
Target: 5'- cGCGUUCGGCgUGAuACGgcgCAcacggaUCAGCCa -3' miRNA: 3'- cCGCGAGUUGgACU-UGUa--GU------AGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 18390 | 0.66 | 0.980879 |
Target: 5'- uGCGCUCGcugccgacguACUUGAaugaaccgccgacgACGUUcgCGGCCg -3' miRNA: 3'- cCGCGAGU----------UGGACU--------------UGUAGuaGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 19036 | 0.66 | 0.970496 |
Target: 5'- cGGCcguuGCUgGAUCUGcgcgcCGUCGUCGAUCg -3' miRNA: 3'- -CCG----CGAgUUGGACuu---GUAGUAGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 19912 | 0.67 | 0.953972 |
Target: 5'- cGGCGCUCAGCagguucGAGCucgaucgcugcugcgCcgCGACCg -3' miRNA: 3'- -CCGCGAGUUGga----CUUGua-------------GuaGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 20226 | 0.67 | 0.963444 |
Target: 5'- cGGCGCaccgaUCAcACCgGAGCcguccUCGUCGAUCa -3' miRNA: 3'- -CCGCG-----AGU-UGGaCUUGu----AGUAGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 23960 | 0.67 | 0.967103 |
Target: 5'- cGGUGCc---CCUGAAUA-CGUCAACUu -3' miRNA: 3'- -CCGCGaguuGGACUUGUaGUAGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 24303 | 0.7 | 0.881464 |
Target: 5'- cGGCGagaUCuuGCUUGAAC-UgAUCGACCg -3' miRNA: 3'- -CCGCg--AGu-UGGACUUGuAgUAGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 24424 | 0.71 | 0.846659 |
Target: 5'- cGGCGCUCAugagcaaaacccGCCgcgucgaUGAACcgC-UCGACCc -3' miRNA: 3'- -CCGCGAGU------------UGG-------ACUUGuaGuAGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 32033 | 0.68 | 0.939307 |
Target: 5'- uGGCgGCUCGGCCgugaacugggGAccgggcgcaccgcgGCGUCgcacGUCAGCCg -3' miRNA: 3'- -CCG-CGAGUUGGa---------CU--------------UGUAG----UAGUUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 34871 | 0.67 | 0.955293 |
Target: 5'- aGCGCcgcCAACgUGAacgagcACGUCAUC-ACCg -3' miRNA: 3'- cCGCGa--GUUGgACU------UGUAGUAGuUGG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 38857 | 0.72 | 0.789483 |
Target: 5'- uGCGCUCGGCCgaacGGGCGUCucgcUCGAgCu -3' miRNA: 3'- cCGCGAGUUGGa---CUUGUAGu---AGUUgG- -5' |
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12198 | 5' | -48.3 | NC_003309.1 | + | 41983 | 0.67 | 0.967103 |
Target: 5'- -aCGUUCAAUa-GGAUAUCGUUAACCc -3' miRNA: 3'- ccGCGAGUUGgaCUUGUAGUAGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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