Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12198 | 5' | -48.3 | NC_003309.1 | + | 16426 | 0.7 | 0.873392 |
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Target: 5'- cGuCGC-CGACCUGcAACGUCAcgucgCGGCCg -3' miRNA: 3'- cC-GCGaGUUGGAC-UUGUAGUa----GUUGG- -5' |
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| 12198 | 5' | -48.3 | NC_003309.1 | + | 12621 | 0.7 | 0.856432 |
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Target: 5'- cGCGCgCGACCUGcuCcgCcUCGGCCg -3' miRNA: 3'- cCGCGaGUUGGACuuGuaGuAGUUGG- -5' |
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| 12198 | 5' | -48.3 | NC_003309.1 | + | 24424 | 0.71 | 0.846659 |
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Target: 5'- cGGCGCUCAugagcaaaacccGCCgcgucgaUGAACcgC-UCGACCc -3' miRNA: 3'- -CCGCGAGU------------UGG-------ACUUGuaGuAGUUGG- -5' |
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| 12198 | 5' | -48.3 | NC_003309.1 | + | 38857 | 0.72 | 0.789483 |
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Target: 5'- uGCGCUCGGCCgaacGGGCGUCucgcUCGAgCu -3' miRNA: 3'- cCGCGAGUUGGa---CUUGUAGu---AGUUgG- -5' |
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| 12198 | 5' | -48.3 | NC_003309.1 | + | 42547 | 0.73 | 0.736032 |
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Target: 5'- uGGCGCUCAGuCCgcaaguUGAGCGccggccgaAUCAGCCa -3' miRNA: 3'- -CCGCGAGUU-GG------ACUUGUag------UAGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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