Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
122 | 5' | -57 | AC_000006.1 | + | 17077 | 0.66 | 0.444395 |
Target: 5'- cGGCCGcguGCCgu-GCCGCGGAgcCAGGGAc -3' miRNA: 3'- aUCGGU---CGGuguCGGUGUCU--GUCUCU- -5' |
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122 | 5' | -57 | AC_000006.1 | + | 11885 | 0.66 | 0.443374 |
Target: 5'- uUAGacaaCAGCCGCAgGCCaACAGACucucggccauucuGGAGGc -3' miRNA: 3'- -AUCg---GUCGGUGU-CGG-UGUCUG-------------UCUCU- -5' |
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122 | 5' | -57 | AC_000006.1 | + | 15218 | 0.66 | 0.433238 |
Target: 5'- gAGCgcggaagUGGCCGCGGaCGCGGACGGGGu -3' miRNA: 3'- aUCG-------GUCGGUGUCgGUGUCUGUCUCu -5' |
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122 | 5' | -57 | AC_000006.1 | + | 10993 | 0.66 | 0.424231 |
Target: 5'- cGGCCaaccuGGUgACGGCCuacgaGCAGACGGuGAa -3' miRNA: 3'- aUCGG-----UCGgUGUCGG-----UGUCUGUCuCU- -5' |
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122 | 5' | -57 | AC_000006.1 | + | 13178 | 0.66 | 0.414356 |
Target: 5'- cGGCCGGCuCACccuGCCGCuGuucACAGAGc -3' miRNA: 3'- aUCGGUCG-GUGu--CGGUGuC---UGUCUCu -5' |
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122 | 5' | -57 | AC_000006.1 | + | 8330 | 0.66 | 0.404623 |
Target: 5'- cGGCggCAGCgGCGGuuccggccCCGCGGGCAGGGGc -3' miRNA: 3'- aUCG--GUCGgUGUC--------GGUGUCUGUCUCU- -5' |
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122 | 5' | -57 | AC_000006.1 | + | 2701 | 0.67 | 0.376309 |
Target: 5'- aAGCCgaaaaAGUCGCAGCCAU--GCAGGGu -3' miRNA: 3'- aUCGG-----UCGGUGUCGGUGucUGUCUCu -5' |
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122 | 5' | -57 | AC_000006.1 | + | 24318 | 0.67 | 0.376309 |
Target: 5'- uUGGCCAGCUgcaagagguaGCAGuggcuCCACAGcgGCGGGGGa -3' miRNA: 3'- -AUCGGUCGG----------UGUC-----GGUGUC--UGUCUCU- -5' |
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122 | 5' | -57 | AC_000006.1 | + | 31092 | 0.68 | 0.332176 |
Target: 5'- cAGCCAGUUugAGUgACAGGacccCAGGGGg -3' miRNA: 3'- aUCGGUCGGugUCGgUGUCU----GUCUCU- -5' |
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122 | 5' | -57 | AC_000006.1 | + | 13187 | 0.69 | 0.295037 |
Target: 5'- cAGCCGccgcgggucaagaacGCCAUGGCCAauaacgGGAUAGAGAg -3' miRNA: 3'- aUCGGU---------------CGGUGUCGGUg-----UCUGUCUCU- -5' |
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122 | 5' | -57 | AC_000006.1 | + | 32972 | 0.69 | 0.291965 |
Target: 5'- aAGCCAGCagCACAugguaauCCACGGACAGAa- -3' miRNA: 3'- aUCGGUCG--GUGUc------GGUGUCUGUCUcu -5' |
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122 | 5' | -57 | AC_000006.1 | + | 6210 | 0.7 | 0.242211 |
Target: 5'- -cGCCAGCCGCGGUUAU--GCAGGGu -3' miRNA: 3'- auCGGUCGGUGUCGGUGucUGUCUCu -5' |
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122 | 5' | -57 | AC_000006.1 | + | 17666 | 0.7 | 0.235709 |
Target: 5'- -uGCCAGuUCugGGCCuggcgGCGGGCGGGGAc -3' miRNA: 3'- auCGGUC-GGugUCGG-----UGUCUGUCUCU- -5' |
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122 | 5' | -57 | AC_000006.1 | + | 17376 | 0.71 | 0.211154 |
Target: 5'- aAG-CGGCCGCGGCCAgAuGCGGAGGa -3' miRNA: 3'- aUCgGUCGGUGUCGGUgUcUGUCUCU- -5' |
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122 | 5' | -57 | AC_000006.1 | + | 10715 | 0.71 | 0.199723 |
Target: 5'- -cGCCuGCUACGGCCGCGGucGCGGuGGu -3' miRNA: 3'- auCGGuCGGUGUCGGUGUC--UGUCuCU- -5' |
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122 | 5' | -57 | AC_000006.1 | + | 3631 | 0.75 | 0.105897 |
Target: 5'- cAGCCgcggcAGCCGCAGCCGCcauGACAGcGAc -3' miRNA: 3'- aUCGG-----UCGGUGUCGGUGu--CUGUCuCU- -5' |
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122 | 5' | -57 | AC_000006.1 | + | 10688 | 1.07 | 0.000388 |
Target: 5'- cUAGCCAGCCACAGCCACAGACAGAGAu -3' miRNA: 3'- -AUCGGUCGGUGUCGGUGUCUGUCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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