Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12203 | 5' | -51.3 | NC_003309.1 | + | 983 | 0.66 | 0.90834 |
Target: 5'- uGCCGAUG--CCGACGccCGCGGCGGuCAc -3' miRNA: 3'- -UGGCUAUagGGCUGCu-GCGUUGUU-GU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 19199 | 0.66 | 0.886896 |
Target: 5'- cGCCGuUGUCCuugcgccauuCGGCGACGUAGCucgucGCGu -3' miRNA: 3'- -UGGCuAUAGG----------GCUGCUGCGUUGu----UGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 47501 | 0.66 | 0.886896 |
Target: 5'- aGCU----UCUCGagGCGGCGCAGCGACAc -3' miRNA: 3'- -UGGcuauAGGGC--UGCUGCGUUGUUGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 33177 | 0.67 | 0.871225 |
Target: 5'- cACUGAUGcgcugaCUGACGAC-CAACGGCAa -3' miRNA: 3'- -UGGCUAUag----GGCUGCUGcGUUGUUGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 8741 | 0.67 | 0.862994 |
Target: 5'- -gCGAUGUCCCGAa-GCGCuuCGAUc -3' miRNA: 3'- ugGCUAUAGGGCUgcUGCGuuGUUGu -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 24018 | 0.67 | 0.862994 |
Target: 5'- cACCG----CCCGucguCGACGCAGCuGCAg -3' miRNA: 3'- -UGGCuauaGGGCu---GCUGCGUUGuUGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 18329 | 0.67 | 0.845781 |
Target: 5'- gACCGG-AUCggacggcauaUCGGcCGACGCGACGACGg -3' miRNA: 3'- -UGGCUaUAG----------GGCU-GCUGCGUUGUUGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 5391 | 0.68 | 0.836816 |
Target: 5'- cACCGAUGUagCCGACGAuCGCGcCAc-- -3' miRNA: 3'- -UGGCUAUAg-GGCUGCU-GCGUuGUugu -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 3559 | 0.68 | 0.827624 |
Target: 5'- gACCGcguaggcggcGUAUCgCGACGacugaucgccGCGCAGCAGCc -3' miRNA: 3'- -UGGC----------UAUAGgGCUGC----------UGCGUUGUUGu -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 44089 | 0.68 | 0.818216 |
Target: 5'- gACCGAUugCCgGGCGAacgUGCAugAGCAc -3' miRNA: 3'- -UGGCUAuaGGgCUGCU---GCGUugUUGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 9703 | 0.68 | 0.808602 |
Target: 5'- uGCCGuGUGUCgCGcGCGAUGC-GCAGCAc -3' miRNA: 3'- -UGGC-UAUAGgGC-UGCUGCGuUGUUGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 12807 | 0.68 | 0.798793 |
Target: 5'- aAUUGAgcuugCCCGACGcCGCgAACGACAc -3' miRNA: 3'- -UGGCUaua--GGGCUGCuGCG-UUGUUGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 32829 | 0.69 | 0.788801 |
Target: 5'- gGCCGAaGUCCUGuGCG--GCAACGACGa -3' miRNA: 3'- -UGGCUaUAGGGC-UGCugCGUUGUUGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 22726 | 0.69 | 0.757843 |
Target: 5'- cGCUGAUcGUCgCCGGCGACGCcgaaAACGc -3' miRNA: 3'- -UGGCUA-UAG-GGCUGCUGCGuug-UUGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 31713 | 0.7 | 0.725668 |
Target: 5'- gGCCGcAUGUCUgGcuCGGCGCGACGAUc -3' miRNA: 3'- -UGGC-UAUAGGgCu-GCUGCGUUGUUGu -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 36083 | 0.7 | 0.725668 |
Target: 5'- gAUCGAcGUCCaCGACGGcCGCGuauACAGCGu -3' miRNA: 3'- -UGGCUaUAGG-GCUGCU-GCGU---UGUUGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 13229 | 0.7 | 0.69261 |
Target: 5'- gACCGAgAUCCCGcCGuuGCGCGcCGGCGa -3' miRNA: 3'- -UGGCUaUAGGGCuGC--UGCGUuGUUGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 9836 | 0.7 | 0.69261 |
Target: 5'- uGCCGAgacaCCCGACaACGCGACcGCc -3' miRNA: 3'- -UGGCUaua-GGGCUGcUGCGUUGuUGu -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 10625 | 0.71 | 0.670244 |
Target: 5'- cGCCGAUuUCCUGACuGC-CGGCAACGu -3' miRNA: 3'- -UGGCUAuAGGGCUGcUGcGUUGUUGU- -5' |
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12203 | 5' | -51.3 | NC_003309.1 | + | 30216 | 0.71 | 0.647727 |
Target: 5'- uGCCGAUugaCCGACGACGaAACAAa- -3' miRNA: 3'- -UGGCUAuagGGCUGCUGCgUUGUUgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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