Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12204 | 3' | -47.6 | NC_003309.1 | + | 5536 | 0.67 | 0.972262 |
Target: 5'- gUCGCcGAccacGAUCUGAUCGAcGUucggauucACGCCg -3' miRNA: 3'- -AGCGuUUa---CUGGACUAGUU-CA--------UGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 5584 | 0.67 | 0.972262 |
Target: 5'- gUCGCGAAUGAacgcCUUGucuUCGcGcGCGCCg -3' miRNA: 3'- -AGCGUUUACU----GGACu--AGUuCaUGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 9509 | 0.69 | 0.929103 |
Target: 5'- gCGCAccGUGACCUGcacgcuugcggcguUCGAGgcaaACGCCg -3' miRNA: 3'- aGCGUu-UACUGGACu-------------AGUUCa---UGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 10536 | 0.7 | 0.907154 |
Target: 5'- uUCGCuucGGUGuACUUGAUCGAGccggccgucaACGCCg -3' miRNA: 3'- -AGCGu--UUAC-UGGACUAGUUCa---------UGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 11143 | 0.73 | 0.761463 |
Target: 5'- uUUGUcGAUGAUCUGAUCGAGcgGUGCCu -3' miRNA: 3'- -AGCGuUUACUGGACUAGUUCa-UGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 12490 | 0.71 | 0.876865 |
Target: 5'- aCGCGauuGAUGGCCUGAUUcAGcucGCGCg -3' miRNA: 3'- aGCGU---UUACUGGACUAGuUCa--UGCGg -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 19122 | 0.72 | 0.79213 |
Target: 5'- uUCG-AAAUGcCCUGAUCGacuaccgAGUACGUCg -3' miRNA: 3'- -AGCgUUUACuGGACUAGU-------UCAUGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 20082 | 0.7 | 0.892598 |
Target: 5'- cUGCAccuUGACCUGAUacg--ACGCCg -3' miRNA: 3'- aGCGUuu-ACUGGACUAguucaUGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 22349 | 0.69 | 0.926705 |
Target: 5'- -aGCcGAUGugCgccGAUCAGGUgcucgacgcaaGCGCCg -3' miRNA: 3'- agCGuUUACugGa--CUAGUUCA-----------UGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 23013 | 0.67 | 0.978049 |
Target: 5'- gCGCgGGAUGAUgUGGUCAgcgAGUcgACGCUu -3' miRNA: 3'- aGCG-UUUACUGgACUAGU---UCA--UGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 25581 | 0.73 | 0.739568 |
Target: 5'- cUUGCAGAUGACCUuuuuaucgaccgGGUCGGGUguagcGgGCCg -3' miRNA: 3'- -AGCGUUUACUGGA------------CUAGUUCA-----UgCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 26492 | 0.66 | 0.984787 |
Target: 5'- aCGCAGA-GGCUUGGcUCGcgcgaacGGcUGCGCCg -3' miRNA: 3'- aGCGUUUaCUGGACU-AGU-------UC-AUGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 32203 | 0.75 | 0.659832 |
Target: 5'- -aGCGAGUG-CC-GAUCAGGU-CGCCg -3' miRNA: 3'- agCGUUUACuGGaCUAGUUCAuGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 33042 | 0.71 | 0.842081 |
Target: 5'- gCGCAcgaucGAcGACCUGAUCAAccGCGCg -3' miRNA: 3'- aGCGU-----UUaCUGGACUAGUUcaUGCGg -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 35858 | 0.67 | 0.978049 |
Target: 5'- gCGCGAacggcaucGUGaACCUGA-CGcAGUACGCa -3' miRNA: 3'- aGCGUU--------UAC-UGGACUaGU-UCAUGCGg -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 48034 | 0.69 | 0.920496 |
Target: 5'- gCGUgaaGAGUGGCC-GGUCGAGguCGCCg -3' miRNA: 3'- aGCG---UUUACUGGaCUAGUUCauGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 49459 | 0.66 | 0.986882 |
Target: 5'- gCGCGAAUG-CCgauugcGAUgCAcGUAUGCCu -3' miRNA: 3'- aGCGUUUACuGGa-----CUA-GUuCAUGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 49674 | 0.66 | 0.982892 |
Target: 5'- cCGCAucucgauuUGACCgucGUCGAGcucgACGCCc -3' miRNA: 3'- aGCGUuu------ACUGGac-UAGUUCa---UGCGG- -5' |
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12204 | 3' | -47.6 | NC_003309.1 | + | 52258 | 0.73 | 0.750582 |
Target: 5'- cCGUcGAcGACUUGAUCGAGUugcCGCCu -3' miRNA: 3'- aGCGuUUaCUGGACUAGUUCAu--GCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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