Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12206 | 3' | -57.8 | NC_003309.1 | + | 7148 | 0.66 | 0.574635 |
Target: 5'- cGAUaccGGCCCgauagcggaUGCGCAUG-CUCGCg -3' miRNA: 3'- cCUAguaCCGGG---------GCGCGUGCaGAGCG- -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 17808 | 0.66 | 0.574635 |
Target: 5'- cGAUCGUcGCCgUGUGCGCGUUcggCGUg -3' miRNA: 3'- cCUAGUAcCGGgGCGCGUGCAGa--GCG- -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 18431 | 0.66 | 0.574635 |
Target: 5'- cGGAUCGUuguaGCUgacgagcgcgaCCGUguaGCGCGUCUUGCg -3' miRNA: 3'- -CCUAGUAc---CGG-----------GGCG---CGUGCAGAGCG- -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 39293 | 0.66 | 0.573574 |
Target: 5'- cGGAcgaugCGUGGCaaCUCGCGCaugcguuGCGUCgccagCGCg -3' miRNA: 3'- -CCUa----GUACCG--GGGCGCG-------UGCAGa----GCG- -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 10059 | 0.67 | 0.553498 |
Target: 5'- ---cCGUGccGCCcgCCGCGCGCGUCagCGCc -3' miRNA: 3'- ccuaGUAC--CGG--GGCGCGUGCAGa-GCG- -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 43985 | 0.67 | 0.553498 |
Target: 5'- --uUCGUGuGCCUuagugcagcgguCGCGCACGcUCUCGg -3' miRNA: 3'- ccuAGUAC-CGGG------------GCGCGUGC-AGAGCg -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 17767 | 0.67 | 0.511987 |
Target: 5'- cGGAUCAgccaGCCguuuuUCGCGCGCGgcagCUCGa -3' miRNA: 3'- -CCUAGUac--CGG-----GGCGCGUGCa---GAGCg -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 19978 | 0.67 | 0.510965 |
Target: 5'- cGGUCGUGaucugcuGCgCCUGCGCGCGaUC-CGCu -3' miRNA: 3'- cCUAGUAC-------CG-GGGCGCGUGC-AGaGCG- -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 28655 | 0.67 | 0.501806 |
Target: 5'- uGAUCAUcGCCaUCGUGUGCGUCUCu- -3' miRNA: 3'- cCUAGUAcCGG-GGCGCGUGCAGAGcg -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 45264 | 0.68 | 0.452341 |
Target: 5'- uGGUCGaaugccGGCCgCCGCGUGCGcCUCGa -3' miRNA: 3'- cCUAGUa-----CCGG-GGCGCGUGCaGAGCg -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 12487 | 0.69 | 0.433309 |
Target: 5'- cGAUUgAUGGCCUgauucagcuCGCGCGCGUUUCa- -3' miRNA: 3'- cCUAG-UACCGGG---------GCGCGUGCAGAGcg -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 10892 | 0.69 | 0.42397 |
Target: 5'- cGGUCGcGGUCgCCGCGCGuucCG-CUCGCu -3' miRNA: 3'- cCUAGUaCCGG-GGCGCGU---GCaGAGCG- -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 37331 | 0.7 | 0.362124 |
Target: 5'- aGAUCAcgGGCgucuaCCUGCGCGCGaUCgugCGCg -3' miRNA: 3'- cCUAGUa-CCG-----GGGCGCGUGC-AGa--GCG- -5' |
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12206 | 3' | -57.8 | NC_003309.1 | + | 11752 | 0.72 | 0.285192 |
Target: 5'- cGAUCA--GCCCCGCcaugcggaccuGCGCGUCguUCGCg -3' miRNA: 3'- cCUAGUacCGGGGCG-----------CGUGCAG--AGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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