Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12209 | 3' | -61.6 | NC_003309.1 | + | 44883 | 0.67 | 0.368873 |
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Target: 5'- cUUGCCGuacacGGCUGACUcagguucgccGGCGG-CGaUCCg -3' miRNA: 3'- -AGCGGC-----CCGACUGA----------CCGCCgGCaAGG- -5' |
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| 12209 | 3' | -61.6 | NC_003309.1 | + | 3634 | 0.67 | 0.35251 |
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Target: 5'- gUCGCCGcccUUGACcGGCGGCaUGUUCUc -3' miRNA: 3'- -AGCGGCcc-GACUGaCCGCCG-GCAAGG- -5' |
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| 12209 | 3' | -61.6 | NC_003309.1 | + | 32390 | 0.67 | 0.344524 |
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Target: 5'- gCGgCGacGGCUcGCUgcGGCGGCCGUgUCCu -3' miRNA: 3'- aGCgGC--CCGAcUGA--CCGCCGGCA-AGG- -5' |
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| 12209 | 3' | -61.6 | NC_003309.1 | + | 37284 | 0.67 | 0.328944 |
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Target: 5'- cUCGCCGGcGCgucgaUGGCGGUCGaggacgUCUa -3' miRNA: 3'- -AGCGGCC-CGacug-ACCGCCGGCa-----AGG- -5' |
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| 12209 | 3' | -61.6 | NC_003309.1 | + | 41300 | 0.68 | 0.3206 |
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Target: 5'- aCGCCugcGGGaUGggaacggcuugacGCUGGCGGCCGggCUc -3' miRNA: 3'- aGCGG---CCCgAC-------------UGACCGCCGGCaaGG- -5' |
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| 12209 | 3' | -61.6 | NC_003309.1 | + | 53309 | 0.69 | 0.271887 |
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Target: 5'- cCGCCGGGCgggcaacGACgGGCgGGCCa--CCa -3' miRNA: 3'- aGCGGCCCGa------CUGaCCG-CCGGcaaGG- -5' |
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| 12209 | 3' | -61.6 | NC_003309.1 | + | 5069 | 0.69 | 0.265341 |
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Target: 5'- aUGCaCGGGC--GC-GGCGGCCGgugCCg -3' miRNA: 3'- aGCG-GCCCGacUGaCCGCCGGCaa-GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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