Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12212 | 3' | -57.6 | NC_003309.1 | + | 11976 | 0.66 | 0.572673 |
Target: 5'- -aGcCGCGGCGcGCGcCGCGUcGGCGUUg -3' miRNA: 3'- uaCaGCGCUGC-CGU-GCGCGcUUGCAA- -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 6760 | 0.66 | 0.572673 |
Target: 5'- uUGaUCGUcACGGCGCGCGCauACGg- -3' miRNA: 3'- uAC-AGCGcUGCCGUGCGCGcuUGCaa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 36556 | 0.66 | 0.561871 |
Target: 5'- -cGUCGCGGcCGGCGCGauCGAgaagGCGa- -3' miRNA: 3'- uaCAGCGCU-GCCGUGCgcGCU----UGCaa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 28819 | 0.66 | 0.55864 |
Target: 5'- cAUGUCGCccGCGGgAuagagcugcaacauCGCGCGAGCGg- -3' miRNA: 3'- -UACAGCGc-UGCCgU--------------GCGCGCUUGCaa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 43503 | 0.66 | 0.551124 |
Target: 5'- -gGUCGCGGCaGUcuccGCgGUGCGAACGg- -3' miRNA: 3'- uaCAGCGCUGcCG----UG-CGCGCUUGCaa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 9719 | 0.66 | 0.540441 |
Target: 5'- -cGUCGCGucgaGCGGUugccgugugucGCGCGCGAuGCGc- -3' miRNA: 3'- uaCAGCGC----UGCCG-----------UGCGCGCU-UGCaa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 8374 | 0.66 | 0.540441 |
Target: 5'- -cGUCGCacgcGCGGCACGCa-GAAUGUUu -3' miRNA: 3'- uaCAGCGc---UGCCGUGCGcgCUUGCAA- -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 49472 | 0.67 | 0.488214 |
Target: 5'- ---aUGCGcCGaGCACGCGCGAAUGc- -3' miRNA: 3'- uacaGCGCuGC-CGUGCGCGCUUGCaa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 19449 | 0.67 | 0.467987 |
Target: 5'- cGUGUCGCGGCGGagccguCACcCGgGAACGc- -3' miRNA: 3'- -UACAGCGCUGCC------GUGcGCgCUUGCaa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 25867 | 0.67 | 0.466988 |
Target: 5'- -cGUCGgGGCGccgaacaGCACcgGCGCGAGCGg- -3' miRNA: 3'- uaCAGCgCUGC-------CGUG--CGCGCUUGCaa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 10684 | 0.68 | 0.458037 |
Target: 5'- cUG-CGCGGCGGC-CGUcucGCGGGCGa- -3' miRNA: 3'- uACaGCGCUGCCGuGCG---CGCUUGCaa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 26407 | 0.68 | 0.448201 |
Target: 5'- -aGUCGuCGAaacaguCGGCAaccgcCGCGCGGACGg- -3' miRNA: 3'- uaCAGC-GCU------GCCGU-----GCGCGCUUGCaa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 10734 | 0.68 | 0.438482 |
Target: 5'- -cGUCGCuGCuGCGCgaGCGCGAGCGa- -3' miRNA: 3'- uaCAGCGcUGcCGUG--CGCGCUUGCaa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 14549 | 0.68 | 0.428886 |
Target: 5'- cUGUUGCaccGACGGCGauuuccugcgccUGCGUGAACGUc -3' miRNA: 3'- uACAGCG---CUGCCGU------------GCGCGCUUGCAa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 15330 | 0.69 | 0.365354 |
Target: 5'- -cGUCgGCGAUGGCugcuGCGCGuCGGAUGUc -3' miRNA: 3'- uaCAG-CGCUGCCG----UGCGC-GCUUGCAa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 22472 | 0.69 | 0.365354 |
Target: 5'- -cGaCGCGGCGGCAuCGUGCGcGGCGa- -3' miRNA: 3'- uaCaGCGCUGCCGU-GCGCGC-UUGCaa -5' |
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12212 | 3' | -57.6 | NC_003309.1 | + | 27936 | 0.73 | 0.204747 |
Target: 5'- -cGUCGCGGCgGGCACGCGCaauuGAuccguCGUUg -3' miRNA: 3'- uaCAGCGCUG-CCGUGCGCG----CUu----GCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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