Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12212 | 5' | -58 | NC_003309.1 | + | 7771 | 0.67 | 0.517525 |
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Target: 5'- cGAAAUaCGGCGCcgGCGCcuUCGcCGGuGCGa -3' miRNA: 3'- cCUUUA-GCCGCG--CGCGu-AGC-GCC-CGU- -5' |
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| 12212 | 5' | -58 | NC_003309.1 | + | 13986 | 0.68 | 0.457238 |
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Target: 5'- aGGAAuUCGGC-CGCaccgaCGaCGCGGGCGg -3' miRNA: 3'- -CCUUuAGCCGcGCGc----GUaGCGCCCGU- -5' |
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| 12212 | 5' | -58 | NC_003309.1 | + | 19288 | 0.68 | 0.447553 |
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Target: 5'- ----uUCGGCGCGCacGCG-CGCGaGGUAu -3' miRNA: 3'- ccuuuAGCCGCGCG--CGUaGCGC-CCGU- -5' |
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| 12212 | 5' | -58 | NC_003309.1 | + | 47339 | 0.7 | 0.357502 |
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Target: 5'- -----gCGGCGCaCGCcgCGCGGGUu -3' miRNA: 3'- ccuuuaGCCGCGcGCGuaGCGCCCGu -5' |
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| 12212 | 5' | -58 | NC_003309.1 | + | 11980 | 0.72 | 0.254171 |
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Target: 5'- cGGcagccgCGGCGCGCGCcgCGUcGGCGu -3' miRNA: 3'- -CCuuua--GCCGCGCGCGuaGCGcCCGU- -5' |
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| 12212 | 5' | -58 | NC_003309.1 | + | 10711 | 0.73 | 0.21462 |
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Target: 5'- cGAAGccUCGGCGCgcucgagcuccugcuGCGCGgcggccgucUCGCGGGCGa -3' miRNA: 3'- cCUUU--AGCCGCG---------------CGCGU---------AGCGCCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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