Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12214 | 3' | -54.6 | NC_003309.1 | + | 12072 | 0.66 | 0.77472 |
Target: 5'- -cGCuACGCGGGCcGccGCGGCGUUGGu -3' miRNA: 3'- gcUGuUGCGCUCGuCa-CGCUGCAGCU- -5' |
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12214 | 3' | -54.6 | NC_003309.1 | + | 22465 | 0.66 | 0.77472 |
Target: 5'- gCGGCAucguGCGCGGcgaucGCAcGcUGcCGGCGUCGAg -3' miRNA: 3'- -GCUGU----UGCGCU-----CGU-C-AC-GCUGCAGCU- -5' |
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12214 | 3' | -54.6 | NC_003309.1 | + | 36365 | 0.66 | 0.754415 |
Target: 5'- cCGACAGCagguCGAcgcGCAGUugaacGCGACGaUCGAa -3' miRNA: 3'- -GCUGUUGc---GCU---CGUCA-----CGCUGC-AGCU- -5' |
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12214 | 3' | -54.6 | NC_003309.1 | + | 30620 | 0.66 | 0.74407 |
Target: 5'- aCGACAaACGCGAGCA---CGACGagCGGc -3' miRNA: 3'- -GCUGU-UGCGCUCGUcacGCUGCa-GCU- -5' |
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12214 | 3' | -54.6 | NC_003309.1 | + | 34555 | 0.67 | 0.690887 |
Target: 5'- gCGcCugguACGCGAGCAGcguaGCGAgcaccCGUCGAu -3' miRNA: 3'- -GCuGu---UGCGCUCGUCa---CGCU-----GCAGCU- -5' |
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12214 | 3' | -54.6 | NC_003309.1 | + | 8848 | 0.68 | 0.669142 |
Target: 5'- aGACGACGCGAacgaacagccgcGCAuucgcuugcguGUGCG-CGUCGu -3' miRNA: 3'- gCUGUUGCGCU------------CGU-----------CACGCuGCAGCu -5' |
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12214 | 3' | -54.6 | NC_003309.1 | + | 7766 | 0.68 | 0.636291 |
Target: 5'- -uACGGCGCcGGCgccuucgccGGUGCGACGcCGAg -3' miRNA: 3'- gcUGUUGCGcUCG---------UCACGCUGCaGCU- -5' |
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12214 | 3' | -54.6 | NC_003309.1 | + | 35051 | 0.68 | 0.62532 |
Target: 5'- cCGAUcccGAC-CGAGCGccuGUGCGACGcCGAc -3' miRNA: 3'- -GCUG---UUGcGCUCGU---CACGCUGCaGCU- -5' |
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12214 | 3' | -54.6 | NC_003309.1 | + | 46088 | 0.68 | 0.62532 |
Target: 5'- gCGACGGCGauAGCAuccucgguGUGgcCGACGUCGAg -3' miRNA: 3'- -GCUGUUGCgcUCGU--------CAC--GCUGCAGCU- -5' |
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12214 | 3' | -54.6 | NC_003309.1 | + | 16917 | 0.71 | 0.48633 |
Target: 5'- cCGACAGCGaCGAGU---GCGGCGcCGAg -3' miRNA: 3'- -GCUGUUGC-GCUCGucaCGCUGCaGCU- -5' |
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12214 | 3' | -54.6 | NC_003309.1 | + | 17734 | 0.71 | 0.476176 |
Target: 5'- uCGAucCGAUGCGAGCGcucGUagaGCGACGUCGu -3' miRNA: 3'- -GCU--GUUGCGCUCGU---CA---CGCUGCAGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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