Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12214 | 5' | -54.2 | NC_003309.1 | + | 3308 | 0.66 | 0.734793 |
Target: 5'- cGCggaaAGGCGCagCGCgUCGAUCAGcgcugCAUCg -3' miRNA: 3'- aCG----UCCGUG--GCG-AGCUAGUCua---GUAG- -5' |
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12214 | 5' | -54.2 | NC_003309.1 | + | 27930 | 0.67 | 0.668704 |
Target: 5'- gGCGGGCACgCGCaaUUGAUCc-GUCGUUg -3' miRNA: 3'- aCGUCCGUG-GCG--AGCUAGucUAGUAG- -5' |
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12214 | 5' | -54.2 | NC_003309.1 | + | 40658 | 0.68 | 0.623765 |
Target: 5'- gGCAGccgcuguucgcuGCACCGCUCcggCGGAUCgAUCu -3' miRNA: 3'- aCGUC------------CGUGGCGAGcuaGUCUAG-UAG- -5' |
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12214 | 5' | -54.2 | NC_003309.1 | + | 44382 | 0.68 | 0.590102 |
Target: 5'- aGUGGGCGCCG-UCGAacgacugcgUCgGGAUCGUCc -3' miRNA: 3'- aCGUCCGUGGCgAGCU---------AG-UCUAGUAG- -5' |
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12214 | 5' | -54.2 | NC_003309.1 | + | 5054 | 0.71 | 0.411559 |
Target: 5'- gGCcGGUGCCGCgacggCGGUCGGAUUcggGUCa -3' miRNA: 3'- aCGuCCGUGGCGa----GCUAGUCUAG---UAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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