Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12217 | 3' | -51.5 | NC_003309.1 | + | 7900 | 0.66 | 0.894805 |
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Target: 5'- cUGCCaAGUcggCAAGCCGAUCggcGUCGg -3' miRNA: 3'- aGCGGcUCAaa-GUUCGGCUGGa--UAGU- -5' |
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| 12217 | 3' | -51.5 | NC_003309.1 | + | 8305 | 0.66 | 0.894073 |
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Target: 5'- uUCGcCCGGGUcgggCAAGCCGAgCUcggauuccuuuucGUCGc -3' miRNA: 3'- -AGC-GGCUCAaa--GUUCGGCUgGA-------------UAGU- -5' |
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| 12217 | 3' | -51.5 | NC_003309.1 | + | 10536 | 0.66 | 0.887362 |
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Target: 5'- uUCGCUucGGUguacuugaUCGAGCCGGCC-GUCAa -3' miRNA: 3'- -AGCGGc-UCAa-------AGUUCGGCUGGaUAGU- -5' |
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| 12217 | 3' | -51.5 | NC_003309.1 | + | 16801 | 0.66 | 0.863398 |
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Target: 5'- cUCGCCGAGa-UUgcGCCGGCCg---- -3' miRNA: 3'- -AGCGGCUCaaAGuuCGGCUGGauagu -5' |
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| 12217 | 3' | -51.5 | NC_003309.1 | + | 5173 | 0.68 | 0.808816 |
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Target: 5'- aUCGCC-AGUUUCGAGgCGAgCUgcgcGUCGc -3' miRNA: 3'- -AGCGGcUCAAAGUUCgGCUgGA----UAGU- -5' |
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| 12217 | 3' | -51.5 | NC_003309.1 | + | 9809 | 0.68 | 0.778729 |
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Target: 5'- cUUGCUGAucGUggugacaagCGGGCCGACCUGUUu -3' miRNA: 3'- -AGCGGCU--CAaa-------GUUCGGCUGGAUAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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