Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12217 | 5' | -51.7 | NC_003309.1 | + | 20867 | 0.69 | 0.73632 |
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Target: 5'- -aCGAGuugauAGUUCGGCgUCGAacCGUCGAu -3' miRNA: 3'- aaGCUCu----UCAAGCCGaAGUU--GCGGCU- -5' |
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| 12217 | 5' | -51.7 | NC_003309.1 | + | 3690 | 0.69 | 0.725383 |
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Target: 5'- -aCGGGAAGUUgcugCGGCUUCGAgGCa-- -3' miRNA: 3'- aaGCUCUUCAA----GCCGAAGUUgCGgcu -5' |
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| 12217 | 5' | -51.7 | NC_003309.1 | + | 553 | 0.7 | 0.658107 |
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Target: 5'- gUCGGGuguuUUCGGCUUU-GCGCCGAc -3' miRNA: 3'- aAGCUCuuc-AAGCCGAAGuUGCGGCU- -5' |
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| 12217 | 5' | -51.7 | NC_003309.1 | + | 10518 | 0.71 | 0.601211 |
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Target: 5'- aUCGAGc----CGGCcgUCAACGCCGAc -3' miRNA: 3'- aAGCUCuucaaGCCGa-AGUUGCGGCU- -5' |
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| 12217 | 5' | -51.7 | NC_003309.1 | + | 4323 | 0.71 | 0.567326 |
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Target: 5'- gUCGAGAuggUUGGCgaUGACGCCGAu -3' miRNA: 3'- aAGCUCUucaAGCCGaaGUUGCGGCU- -5' |
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| 12217 | 5' | -51.7 | NC_003309.1 | + | 4222 | 0.72 | 0.52735 |
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Target: 5'- -cCGAGAAGUugacgauugcggugaUCGGCUUCAccaacgaggcaGCGCgGAu -3' miRNA: 3'- aaGCUCUUCA---------------AGCCGAAGU-----------UGCGgCU- -5' |
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| 12217 | 5' | -51.7 | NC_003309.1 | + | 51526 | 0.76 | 0.320442 |
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Target: 5'- gUCGAGGAaagccaUCGGCUUCGGCGCCc- -3' miRNA: 3'- aAGCUCUUca----AGCCGAAGUUGCGGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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