miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12217 5' -51.7 NC_003309.1 + 51526 0.76 0.320442
Target:  5'- gUCGAGGAaagccaUCGGCUUCGGCGCCc- -3'
miRNA:   3'- aAGCUCUUca----AGCCGAAGUUGCGGcu -5'
12217 5' -51.7 NC_003309.1 + 4222 0.72 0.52735
Target:  5'- -cCGAGAAGUugacgauugcggugaUCGGCUUCAccaacgaggcaGCGCgGAu -3'
miRNA:   3'- aaGCUCUUCA---------------AGCCGAAGU-----------UGCGgCU- -5'
12217 5' -51.7 NC_003309.1 + 10518 0.71 0.601211
Target:  5'- aUCGAGc----CGGCcgUCAACGCCGAc -3'
miRNA:   3'- aAGCUCuucaaGCCGa-AGUUGCGGCU- -5'
12217 5' -51.7 NC_003309.1 + 4323 0.71 0.567326
Target:  5'- gUCGAGAuggUUGGCgaUGACGCCGAu -3'
miRNA:   3'- aAGCUCUucaAGCCGaaGUUGCGGCU- -5'
12217 5' -51.7 NC_003309.1 + 553 0.7 0.658107
Target:  5'- gUCGGGuguuUUCGGCUUU-GCGCCGAc -3'
miRNA:   3'- aAGCUCuuc-AAGCCGAAGuUGCGGCU- -5'
12217 5' -51.7 NC_003309.1 + 20867 0.69 0.73632
Target:  5'- -aCGAGuugauAGUUCGGCgUCGAacCGUCGAu -3'
miRNA:   3'- aaGCUCu----UCAAGCCGaAGUU--GCGGCU- -5'
12217 5' -51.7 NC_003309.1 + 3690 0.69 0.725383
Target:  5'- -aCGGGAAGUUgcugCGGCUUCGAgGCa-- -3'
miRNA:   3'- aaGCUCUUCAA----GCCGAAGUUgCGgcu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.