Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12218 | 3' | -57.1 | NC_003309.1 | + | 43970 | 0.66 | 0.633531 |
Target: 5'- gCAGCGG-UCGCGCAcGCUcucGgGCaUCGGg -3' miRNA: 3'- -GUCGCCuAGCGCGU-UGA---CgCGcAGCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 11973 | 0.66 | 0.633531 |
Target: 5'- -cGCGGcgCGCGCcGCgucgGCGUUGAa -3' miRNA: 3'- guCGCCuaGCGCGuUGacg-CGCAGCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 18149 | 0.66 | 0.632442 |
Target: 5'- aCAGUGa--CGCGCGAUUGCGUacagcgcccacuuGUCGAc -3' miRNA: 3'- -GUCGCcuaGCGCGUUGACGCG-------------CAGCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 50282 | 0.66 | 0.600884 |
Target: 5'- --aUGGcUCGCGCcgaAACUGCGC-UCGAg -3' miRNA: 3'- gucGCCuAGCGCG---UUGACGCGcAGCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 11233 | 0.66 | 0.600884 |
Target: 5'- -cGCGGAcUGCGCGucguCUGCcGCGcgCGAc -3' miRNA: 3'- guCGCCUaGCGCGUu---GACG-CGCa-GCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 9097 | 0.67 | 0.568471 |
Target: 5'- uCGGCGGAcgacaucugcUCGCGCAGCUcgGCuaaCGUCu- -3' miRNA: 3'- -GUCGCCU----------AGCGCGUUGA--CGc--GCAGcu -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 17812 | 0.67 | 0.568471 |
Target: 5'- uCGGC-GAUCGuCGcCGugUGCGCGUuCGGc -3' miRNA: 3'- -GUCGcCUAGC-GC-GUugACGCGCA-GCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 27701 | 0.67 | 0.536512 |
Target: 5'- uCAGCGGGUaacgUGgGCugAACUGCGCGaaaaauggcgucUCGAg -3' miRNA: 3'- -GUCGCCUA----GCgCG--UUGACGCGC------------AGCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 5181 | 0.68 | 0.494937 |
Target: 5'- -cGCGcucGAUCGCcaguuucgagGCgAGCUGCGCGUCGc -3' miRNA: 3'- guCGC---CUAGCG----------CG-UUGACGCGCAGCu -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 27932 | 0.68 | 0.484768 |
Target: 5'- gCGGCGGGcaCGCGCAAUUGaucCGUCGu -3' miRNA: 3'- -GUCGCCUa-GCGCGUUGACgc-GCAGCu -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 8828 | 0.68 | 0.46474 |
Target: 5'- -cGCGcauUCGCuuGCGugUGCGCGUCGu -3' miRNA: 3'- guCGCcu-AGCG--CGUugACGCGCAGCu -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 4990 | 0.69 | 0.454888 |
Target: 5'- uCAGCGGcuucCGCGCGuucgauCUGCGCGgUCa- -3' miRNA: 3'- -GUCGCCua--GCGCGUu-----GACGCGC-AGcu -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 37311 | 0.69 | 0.449032 |
Target: 5'- -cGCGcGAUCGUGCGcgacGCccugaaucucgccgGCGCGUCGAu -3' miRNA: 3'- guCGC-CUAGCGCGU----UGa-------------CGCGCAGCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 37101 | 0.69 | 0.449032 |
Target: 5'- gCAGCGacgcaGAUCGCGCAgcagaaggagaacgaACUGCGUGcagcCGAg -3' miRNA: 3'- -GUCGC-----CUAGCGCGU---------------UGACGCGCa---GCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 37238 | 0.69 | 0.435531 |
Target: 5'- aGGCGG--CGCuaCAGCaGCGCGUCGAg -3' miRNA: 3'- gUCGCCuaGCGc-GUUGaCGCGCAGCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 17534 | 0.69 | 0.426032 |
Target: 5'- --cUGGAUCugGCGCAcccACgGCGCGUCGGa -3' miRNA: 3'- gucGCCUAG--CGCGU---UGaCGCGCAGCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 46490 | 0.69 | 0.426032 |
Target: 5'- uCGGCGGGaacugcUCGCGCAua-GCGCgGUUGAg -3' miRNA: 3'- -GUCGCCU------AGCGCGUugaCGCG-CAGCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 9559 | 0.69 | 0.41387 |
Target: 5'- gCGGCGGcUUGUGCGuauucgcggaucgaACUGCGCGccgcgUCGAg -3' miRNA: 3'- -GUCGCCuAGCGCGU--------------UGACGCGC-----AGCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 3308 | 0.69 | 0.407411 |
Target: 5'- -cGCGGAaagGCGCA---GCGCGUCGAu -3' miRNA: 3'- guCGCCUag-CGCGUugaCGCGCAGCU- -5' |
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12218 | 3' | -57.1 | NC_003309.1 | + | 15334 | 0.69 | 0.407411 |
Target: 5'- gCAGCGucggCGaugGCuGCUGCGCGUCGGa -3' miRNA: 3'- -GUCGCcua-GCg--CGuUGACGCGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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