miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12219 5' -60 NC_003309.1 + 5078 0.66 0.435081
Target:  5'- -cGGUUGUGCaugcaCGGGCGCGGCg---- -3'
miRNA:   3'- guCCAGCGCGc----GCUCGCGCCGauaga -5'
12219 5' -60 NC_003309.1 + 10734 0.67 0.398393
Target:  5'- --cGUCGCugcuGCGCGAGCGCGaGCg---- -3'
miRNA:   3'- gucCAGCG----CGCGCUCGCGC-CGauaga -5'
12219 5' -60 NC_003309.1 + 11833 0.67 0.380809
Target:  5'- -uGGuUCGCGCGCccGcCGCGGCUuUCg -3'
miRNA:   3'- guCC-AGCGCGCGcuC-GCGCCGAuAGa -5'
12219 5' -60 NC_003309.1 + 9696 0.69 0.293701
Target:  5'- --uGUCGCGCGCGAuGCGCaGCacGUCg -3'
miRNA:   3'- gucCAGCGCGCGCU-CGCGcCGa-UAGa -5'
12219 5' -60 NC_003309.1 + 15435 0.69 0.293701
Target:  5'- gAGGUaGCGCGCGAGUGUccGGCg---- -3'
miRNA:   3'- gUCCAgCGCGCGCUCGCG--CCGauaga -5'
12219 5' -60 NC_003309.1 + 45886 0.69 0.293701
Target:  5'- uCAGcUCGCGCGCGuugaucacgcGGCGaCGGCcGUCg -3'
miRNA:   3'- -GUCcAGCGCGCGC----------UCGC-GCCGaUAGa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.