Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12219 | 5' | -60 | NC_003309.1 | + | 5078 | 0.66 | 0.435081 |
Target: 5'- -cGGUUGUGCaugcaCGGGCGCGGCg---- -3' miRNA: 3'- guCCAGCGCGc----GCUCGCGCCGauaga -5' |
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12219 | 5' | -60 | NC_003309.1 | + | 10734 | 0.67 | 0.398393 |
Target: 5'- --cGUCGCugcuGCGCGAGCGCGaGCg---- -3' miRNA: 3'- gucCAGCG----CGCGCUCGCGC-CGauaga -5' |
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12219 | 5' | -60 | NC_003309.1 | + | 11833 | 0.67 | 0.380809 |
Target: 5'- -uGGuUCGCGCGCccGcCGCGGCUuUCg -3' miRNA: 3'- guCC-AGCGCGCGcuC-GCGCCGAuAGa -5' |
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12219 | 5' | -60 | NC_003309.1 | + | 9696 | 0.69 | 0.293701 |
Target: 5'- --uGUCGCGCGCGAuGCGCaGCacGUCg -3' miRNA: 3'- gucCAGCGCGCGCU-CGCGcCGa-UAGa -5' |
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12219 | 5' | -60 | NC_003309.1 | + | 15435 | 0.69 | 0.293701 |
Target: 5'- gAGGUaGCGCGCGAGUGUccGGCg---- -3' miRNA: 3'- gUCCAgCGCGCGCUCGCG--CCGauaga -5' |
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12219 | 5' | -60 | NC_003309.1 | + | 45886 | 0.69 | 0.293701 |
Target: 5'- uCAGcUCGCGCGCGuugaucacgcGGCGaCGGCcGUCg -3' miRNA: 3'- -GUCcAGCGCGCGC----------UCGC-GCCGaUAGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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