Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12220 | 3' | -54.1 | NC_003309.1 | + | 11982 | 0.66 | 0.789905 |
Target: 5'- cGCGGCAG---CCgCGGCGCGcGCcgcgUCGg -3' miRNA: 3'- -CGCUGUCauaGG-GCCGCGU-CGaa--AGC- -5' |
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12220 | 3' | -54.1 | NC_003309.1 | + | 43500 | 0.66 | 0.779942 |
Target: 5'- cGCGGCAGUcUCCgCGGUGCgaacggucaAGCcaauccaUUCGg -3' miRNA: 3'- -CGCUGUCAuAGG-GCCGCG---------UCGa------AAGC- -5' |
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12220 | 3' | -54.1 | NC_003309.1 | + | 11840 | 0.66 | 0.769824 |
Target: 5'- uCGAUguuGGUucgcgcgCCCGcCGCGGCUUUCGa -3' miRNA: 3'- cGCUG---UCAua-----GGGCcGCGUCGAAAGC- -5' |
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12220 | 3' | -54.1 | NC_003309.1 | + | 19362 | 0.66 | 0.769824 |
Target: 5'- gGCGGCGGagugguuuUCCCGGU-CAGCUgcguaUCGa -3' miRNA: 3'- -CGCUGUCau------AGGGCCGcGUCGAa----AGC- -5' |
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12220 | 3' | -54.1 | NC_003309.1 | + | 44248 | 0.66 | 0.75542 |
Target: 5'- uCGACAGUcgaaugugcgcccgGUCCCgucugccaaccGGCGaccgcuaCAGCUUUCGg -3' miRNA: 3'- cGCUGUCA--------------UAGGG-----------CCGC-------GUCGAAAGC- -5' |
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12220 | 3' | -54.1 | NC_003309.1 | + | 11561 | 0.66 | 0.749168 |
Target: 5'- uCGGCAGcGUCCCG-UGCGGCcacgUUCa -3' miRNA: 3'- cGCUGUCaUAGGGCcGCGUCGa---AAGc -5' |
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12220 | 3' | -54.1 | NC_003309.1 | + | 15996 | 0.69 | 0.595099 |
Target: 5'- cGCGAauugGUCCguCGGCGCGGCUgcgagguUUCGg -3' miRNA: 3'- -CGCUgucaUAGG--GCCGCGUCGA-------AAGC- -5' |
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12220 | 3' | -54.1 | NC_003309.1 | + | 18652 | 0.69 | 0.585181 |
Target: 5'- gGCGAUcaccUGUCCCGGCGCacaaAGCgucccgUCGa -3' miRNA: 3'- -CGCUGuc--AUAGGGCCGCG----UCGaa----AGC- -5' |
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12220 | 3' | -54.1 | NC_003309.1 | + | 38132 | 0.7 | 0.545894 |
Target: 5'- cCGACAgccGUAUCCCGcggcaauugcuccgcGCGCAGUucUUUCGa -3' miRNA: 3'- cGCUGU---CAUAGGGC---------------CGCGUCG--AAAGC- -5' |
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12220 | 3' | -54.1 | NC_003309.1 | + | 46083 | 0.7 | 0.509595 |
Target: 5'- gGCGAUAGcAUCCuCGGUGUGGCcgacgUCGa -3' miRNA: 3'- -CGCUGUCaUAGG-GCCGCGUCGaa---AGC- -5' |
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12220 | 3' | -54.1 | NC_003309.1 | + | 37628 | 0.72 | 0.448236 |
Target: 5'- uGCGAUAacGUCCCGGC-CGGCUacgUCGg -3' miRNA: 3'- -CGCUGUcaUAGGGCCGcGUCGAa--AGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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