Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12222 | 5' | -52 | NC_003309.1 | + | 7901 | 0.66 | 0.901232 |
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Target: 5'- aCUGCCaagucggcaagccgaUCGGCGUCGgUGAACg--UCGGc -3' miRNA: 3'- -GAUGG---------------AGCUGCGGC-ACUUGaaaGGCC- -5' |
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| 12222 | 5' | -52 | NC_003309.1 | + | 12799 | 0.66 | 0.891302 |
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Target: 5'- uUGCC-CGACGCCGcGAACgacaCGa -3' miRNA: 3'- gAUGGaGCUGCGGCaCUUGaaagGCc -5' |
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| 12222 | 5' | -52 | NC_003309.1 | + | 47608 | 0.66 | 0.876209 |
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Target: 5'- cCUGCCcggUCGucgccGCGCCGUccgGCUUUaCCGGg -3' miRNA: 3'- -GAUGG---AGC-----UGCGGCAcu-UGAAA-GGCC- -5' |
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| 12222 | 5' | -52 | NC_003309.1 | + | 22716 | 0.67 | 0.851664 |
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Target: 5'- -cGCCggCGACGCCGaaAACgcgUUCCGc -3' miRNA: 3'- gaUGGa-GCUGCGGCacUUGa--AAGGCc -5' |
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| 12222 | 5' | -52 | NC_003309.1 | + | 37319 | 0.68 | 0.819453 |
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Target: 5'- uCUACCUgcgcgcgaucgugcgCGACGCCcUGAAuCUcgCCGGc -3' miRNA: 3'- -GAUGGA---------------GCUGCGGcACUU-GAaaGGCC- -5' |
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| 12222 | 5' | -52 | NC_003309.1 | + | 24180 | 0.68 | 0.810025 |
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Target: 5'- aUACCUCGucgugcguagggccaACGCCGcc-GCUUUCCGc -3' miRNA: 3'- gAUGGAGC---------------UGCGGCacuUGAAAGGCc -5' |
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| 12222 | 5' | -52 | NC_003309.1 | + | 32036 | 0.69 | 0.745658 |
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Target: 5'- -cGCUggCGGCucgGCCGUGAACUggggaCCGGg -3' miRNA: 3'- gaUGGa-GCUG---CGGCACUUGAaa---GGCC- -5' |
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| 12222 | 5' | -52 | NC_003309.1 | + | 28774 | 0.7 | 0.710403 |
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Target: 5'- -aGCaUUCGACGUCGUGAGCcacucuucccaaucgUCCGGc -3' miRNA: 3'- gaUG-GAGCUGCGGCACUUGaa-------------AGGCC- -5' |
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| 12222 | 5' | -52 | NC_003309.1 | + | 39029 | 0.74 | 0.474645 |
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Target: 5'- uUGCCUCGACGCaaucGAGCUcaugccgUCCGGc -3' miRNA: 3'- gAUGGAGCUGCGgca-CUUGAa------AGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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