Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12224 | 3' | -55.5 | NC_003309.1 | + | 22598 | 0.66 | 0.609888 |
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Target: 5'- ----cGUGCaguGUCGGaCAGCGAcGCGCAGc -3' miRNA: 3'- aaguuCAUG---CAGCC-GUCGCU-CGCGUU- -5' |
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| 12224 | 3' | -55.5 | NC_003309.1 | + | 33632 | 0.66 | 0.598612 |
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Target: 5'- aUCGAGcccucaGUCGGCAGCcGGgGCGAc -3' miRNA: 3'- aAGUUCaug---CAGCCGUCGcUCgCGUU- -5' |
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| 12224 | 3' | -55.5 | NC_003309.1 | + | 5336 | 0.68 | 0.521027 |
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Target: 5'- gUUCGAGa--G-CGGCAGCGuGCGCGc -3' miRNA: 3'- -AAGUUCaugCaGCCGUCGCuCGCGUu -5' |
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| 12224 | 3' | -55.5 | NC_003309.1 | + | 43602 | 0.69 | 0.418017 |
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Target: 5'- -cCGGGUGCccGUCgGGCAGguaCGAGCGCAGg -3' miRNA: 3'- aaGUUCAUG--CAG-CCGUC---GCUCGCGUU- -5' |
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| 12224 | 3' | -55.5 | NC_003309.1 | + | 45237 | 0.75 | 0.19907 |
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Target: 5'- cUCGAaUACGUCGGCGGCacuccAGCGCAGa -3' miRNA: 3'- aAGUUcAUGCAGCCGUCGc----UCGCGUU- -5' |
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| 12224 | 3' | -55.5 | NC_003309.1 | + | 44865 | 0.76 | 0.154929 |
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Target: 5'- cUCAGGUuCGcCGGCGGCGAuccGCGCAAg -3' miRNA: 3'- aAGUUCAuGCaGCCGUCGCU---CGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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