Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12224 | 5' | -52.4 | NC_003309.1 | + | 27514 | 0.66 | 0.85954 |
Target: 5'- -uUCGCGCUCGacCAGUUAaAGCGcAUCc -3' miRNA: 3'- ccAGCGCGAGCa-GUCGAUgUUGC-UAG- -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 43178 | 0.66 | 0.851018 |
Target: 5'- cGUCGCGCgaaccgCGUCAGCgagccCGAg- -3' miRNA: 3'- cCAGCGCGa-----GCAGUCGauguuGCUag -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 22381 | 0.66 | 0.845789 |
Target: 5'- cGUCGaacgcccgcgcggccUGCUCGUCGGCaagccgaugUGCGcCGAUCa -3' miRNA: 3'- cCAGC---------------GCGAGCAGUCG---------AUGUuGCUAG- -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 44889 | 0.66 | 0.845789 |
Target: 5'- -cUCGCGCUUGccguacaCGGCUgacucagguucgccgGCGGCGAUCc -3' miRNA: 3'- ccAGCGCGAGCa------GUCGA---------------UGUUGCUAG- -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 12540 | 0.66 | 0.842257 |
Target: 5'- -uUCGCGCgcccuUCGUCAGUUcgucccgucgACGGCGAa- -3' miRNA: 3'- ccAGCGCG-----AGCAGUCGA----------UGUUGCUag -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 44257 | 0.66 | 0.842257 |
Target: 5'- cGGUUGUGCUCGaCAGUcgaaugUGCGccCGGUCc -3' miRNA: 3'- -CCAGCGCGAGCaGUCG------AUGUu-GCUAG- -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 46284 | 0.66 | 0.83689 |
Target: 5'- --gCGCGCgugcccuuccuugcCGUCAGCgGCAuCGAUCa -3' miRNA: 3'- ccaGCGCGa-------------GCAGUCGaUGUuGCUAG- -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 10703 | 0.66 | 0.835083 |
Target: 5'- cGG-CGCGCUCGagcuccugcugcgCGGCggccgucucgcggGCGAUGAUCg -3' miRNA: 3'- -CCaGCGCGAGCa------------GUCGa------------UGUUGCUAG- -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 12166 | 0.66 | 0.833267 |
Target: 5'- cGGUUGCGaccgcCUCGacgcgCAGUUGC-GCGAUCc -3' miRNA: 3'- -CCAGCGC-----GAGCa----GUCGAUGuUGCUAG- -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 50455 | 0.67 | 0.824058 |
Target: 5'- uGGUCGCcaucuuaccGCUCGUCAGCggucCAcUGAa- -3' miRNA: 3'- -CCAGCG---------CGAGCAGUCGau--GUuGCUag -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 44178 | 0.67 | 0.824058 |
Target: 5'- cGG-CGCGCUgcCGcUCGGCguacGCGACGAg- -3' miRNA: 3'- -CCaGCGCGA--GC-AGUCGa---UGUUGCUag -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 16754 | 0.67 | 0.814639 |
Target: 5'- cGUCGCGCgccgaCGUCAGgaACGcGCGGa- -3' miRNA: 3'- cCAGCGCGa----GCAGUCgaUGU-UGCUag -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 11447 | 0.67 | 0.795217 |
Target: 5'- cGUCGcCGC-CGUCuGUUGCGgacacgaaGCGGUCa -3' miRNA: 3'- cCAGC-GCGaGCAGuCGAUGU--------UGCUAG- -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 4147 | 0.68 | 0.775088 |
Target: 5'- cGGUCGCGCUCccgCcGUUGCuguCGAUg -3' miRNA: 3'- -CCAGCGCGAGca-GuCGAUGuu-GCUAg -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 39959 | 0.68 | 0.742711 |
Target: 5'- cGG-CGCGCUUGUCGagcaccuGUUGCGcgaaguugcaaGCGAUCu -3' miRNA: 3'- -CCaGCGCGAGCAGU-------CGAUGU-----------UGCUAG- -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 20297 | 0.69 | 0.689405 |
Target: 5'- -aUCGCGUUCGUgaucCAGCccGCAcCGAUCa -3' miRNA: 3'- ccAGCGCGAGCA----GUCGa-UGUuGCUAG- -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 36357 | 0.69 | 0.689405 |
Target: 5'- aGGUCGaCGCg---CAGUUgaacGCGACGAUCg -3' miRNA: 3'- -CCAGC-GCGagcaGUCGA----UGUUGCUAG- -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 43707 | 0.7 | 0.611191 |
Target: 5'- --gCGUGCcaUCGUCGGCUaGCAGCGcgCg -3' miRNA: 3'- ccaGCGCG--AGCAGUCGA-UGUUGCuaG- -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 10049 | 0.71 | 0.60001 |
Target: 5'- cGcCGCGCgCGUCAGCgccuCGACGAg- -3' miRNA: 3'- cCaGCGCGaGCAGUCGau--GUUGCUag -5' |
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12224 | 5' | -52.4 | NC_003309.1 | + | 8708 | 0.74 | 0.430355 |
Target: 5'- cGGUCGC-CgacCGUCGGCUcgcGCAcuGCGAUCu -3' miRNA: 3'- -CCAGCGcGa--GCAGUCGA---UGU--UGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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