Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12225 | 3' | -53.9 | NC_003309.1 | + | 45876 | 0.77 | 0.221912 |
Target: 5'- cGCGuuGAUCacgCGGCGACGGCCgucGAUCACGAg -3' miRNA: 3'- -CGU--CUAG---GUCGUUGCCGG---CUAGUGCU- -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 43639 | 0.69 | 0.5793 |
Target: 5'- cGCAGcgUCGGUuccUGGCCGAucUCGCGGu -3' miRNA: 3'- -CGUCuaGGUCGuu-GCCGGCU--AGUGCU- -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 37237 | 0.67 | 0.743895 |
Target: 5'- gGCGGcgCuaCAGCAGCGcGUCGAggACGAg -3' miRNA: 3'- -CGUCuaG--GUCGUUGC-CGGCUagUGCU- -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 34096 | 0.72 | 0.442687 |
Target: 5'- uGCAGGUCUA-CGGCGuGCCGAaggUCugGAa -3' miRNA: 3'- -CGUCUAGGUcGUUGC-CGGCU---AGugCU- -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 32935 | 0.71 | 0.47684 |
Target: 5'- aGCuGAUCgCGGCAagcaacaacggcuauGCGGCCGGacugCGCGAc -3' miRNA: 3'- -CGuCUAG-GUCGU---------------UGCCGGCUa---GUGCU- -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 23479 | 0.76 | 0.246589 |
Target: 5'- gGCGGAUCaagguagaaCAGCGuguGCGGCCGAUCguaACGAu -3' miRNA: 3'- -CGUCUAG---------GUCGU---UGCCGGCUAG---UGCU- -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 18666 | 0.69 | 0.623577 |
Target: 5'- uUAGggUCGGCAACGGC-GAUCACc- -3' miRNA: 3'- cGUCuaGGUCGUUGCCGgCUAGUGcu -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 18327 | 0.72 | 0.432906 |
Target: 5'- cCGGAUCggaCGGCAuauCGGCCGA-CGCGAc -3' miRNA: 3'- cGUCUAG---GUCGUu--GCCGGCUaGUGCU- -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 15335 | 0.72 | 0.452591 |
Target: 5'- cGCAGcgUCGGCGAUGGCUGcugCGCGu -3' miRNA: 3'- -CGUCuaGGUCGUUGCCGGCua-GUGCu -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 15019 | 0.68 | 0.6458 |
Target: 5'- aCGGAaggUCAGCGACGGCCGccccuUCgACGGc -3' miRNA: 3'- cGUCUa--GGUCGUUGCCGGCu----AG-UGCU- -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 11075 | 0.67 | 0.743895 |
Target: 5'- cGCGGAa-CAGCAaccaguucGCGGCCGcgC-CGAg -3' miRNA: 3'- -CGUCUagGUCGU--------UGCCGGCuaGuGCU- -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 10693 | 0.67 | 0.7333 |
Target: 5'- -gAGcUCCugcugcGCGGCGGCCGucUCGCGGg -3' miRNA: 3'- cgUCuAGGu-----CGUUGCCGGCu-AGUGCU- -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 8624 | 0.68 | 0.667972 |
Target: 5'- aGCGGGUUCAGCAccgcugcacGCaGGCUcGUCACGc -3' miRNA: 3'- -CGUCUAGGUCGU---------UG-CCGGcUAGUGCu -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 4177 | 0.74 | 0.359466 |
Target: 5'- cGCGGAUgucguccgCCAGCucgAACGcGCCGGUCGCGc -3' miRNA: 3'- -CGUCUA--------GGUCG---UUGC-CGGCUAGUGCu -5' |
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12225 | 3' | -53.9 | NC_003309.1 | + | 3330 | 0.66 | 0.774939 |
Target: 5'- uGUAGAUCCGcGCGAUG-UCGAgCGCGGa -3' miRNA: 3'- -CGUCUAGGU-CGUUGCcGGCUaGUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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