Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12225 | 5' | -55.3 | NC_003309.1 | + | 18550 | 0.66 | 0.726496 |
Target: 5'- --aCGGUGC-GCCugCGCCugcgacgugcaGcCGAACg -3' miRNA: 3'- gaaGCCAUGuCGGugGCGG-----------CaGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 5052 | 0.66 | 0.683444 |
Target: 5'- --cCGGUGCcGCgACgGCgGUCGGAUu -3' miRNA: 3'- gaaGCCAUGuCGgUGgCGgCAGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 48916 | 0.66 | 0.683444 |
Target: 5'- --aCGGUucgccGCGGCaCGCCGCCaaauacuUCGAACc -3' miRNA: 3'- gaaGCCA-----UGUCG-GUGGCGGc------AGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 1259 | 0.66 | 0.683444 |
Target: 5'- uUUC-GUACAGCgCGgCGCUGUCGuGCu -3' miRNA: 3'- gAAGcCAUGUCG-GUgGCGGCAGCuUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 18750 | 0.66 | 0.683444 |
Target: 5'- --gCGGcgAUAGUCGgCGCCuuGUCGAGCg -3' miRNA: 3'- gaaGCCa-UGUCGGUgGCGG--CAGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 44398 | 0.66 | 0.683444 |
Target: 5'- -cUCGGggaaguUGCaaAGUgGgCGCCGUCGAACg -3' miRNA: 3'- gaAGCC------AUG--UCGgUgGCGGCAGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 1777 | 0.67 | 0.671438 |
Target: 5'- --gCGGcGCAacgcagaaguaauGCCGCCGCCcGUCGAu- -3' miRNA: 3'- gaaGCCaUGU-------------CGGUGGCGG-CAGCUug -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 43697 | 0.67 | 0.66158 |
Target: 5'- -gUCGGcuaGCAGCgCGCgCGCCaGaUCGAACa -3' miRNA: 3'- gaAGCCa--UGUCG-GUG-GCGG-C-AGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 47424 | 0.67 | 0.66158 |
Target: 5'- --gCGGUGCucgcaccagucgAGCCACgCGUCGaCGGGCa -3' miRNA: 3'- gaaGCCAUG------------UCGGUG-GCGGCaGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 26425 | 0.67 | 0.650601 |
Target: 5'- aUUCgGGUACAG-CACaguaGUCGUCGAAa -3' miRNA: 3'- gAAG-CCAUGUCgGUGg---CGGCAGCUUg -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 7835 | 0.67 | 0.649502 |
Target: 5'- --cCGGaACGGCCGCCagucaGCCcggcgagcgccauGUCGAGCg -3' miRNA: 3'- gaaGCCaUGUCGGUGG-----CGG-------------CAGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 8726 | 0.67 | 0.639606 |
Target: 5'- gCUUCGauccagaacaUGCGGUCGCCGaCCGUCGGcucGCg -3' miRNA: 3'- -GAAGCc---------AUGUCGGUGGC-GGCAGCU---UG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 34485 | 0.67 | 0.639606 |
Target: 5'- -aUCGGcUGCAcGCCGCagaCGCUGUUGGAUu -3' miRNA: 3'- gaAGCC-AUGU-CGGUG---GCGGCAGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 22453 | 0.68 | 0.606618 |
Target: 5'- --gCGGcgaucGCAcGCUGCCGgCGUCGAGCg -3' miRNA: 3'- gaaGCCa----UGU-CGGUGGCgGCAGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 44481 | 0.68 | 0.573826 |
Target: 5'- --cCGGU-CGuGCCGCUGCCGaagaacggaUCGAACa -3' miRNA: 3'- gaaGCCAuGU-CGGUGGCGGC---------AGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 15329 | 0.71 | 0.439065 |
Target: 5'- -gUCGGcgAUGGCUGCUGCgCGUCGGAUg -3' miRNA: 3'- gaAGCCa-UGUCGGUGGCG-GCAGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 3952 | 0.71 | 0.437127 |
Target: 5'- -gUCGGcgcuccgaugcgGCAGCCaucugcgcGCCGCUGUCGAAUu -3' miRNA: 3'- gaAGCCa-----------UGUCGG--------UGGCGGCAGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 27206 | 0.72 | 0.383178 |
Target: 5'- --gCGGcGCAGCCacGCUGCCGUUGAuucGCg -3' miRNA: 3'- gaaGCCaUGUCGG--UGGCGGCAGCU---UG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 45201 | 0.72 | 0.365628 |
Target: 5'- --gCGGUAauugaaaaGGCCGCCGCCGaccaccUCGAAUg -3' miRNA: 3'- gaaGCCAUg-------UCGGUGGCGGC------AGCUUG- -5' |
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12225 | 5' | -55.3 | NC_003309.1 | + | 22494 | 0.74 | 0.265457 |
Target: 5'- -gUUGGUcgacgGCGGCCACCGCCGaCGcGGCg -3' miRNA: 3'- gaAGCCA-----UGUCGGUGGCGGCaGC-UUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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