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miRNA: 3'- gAAGC-CA------UGUCGGUG-GCGGCAGCU--UG- -5'
miRNA: 3'- -GAAGCCauguCGGUGGCG-GCAGCUUG- -5'
miRNA: 3'- -GAAGCC----AUG-UCGGUGGCggCAGCUUG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 12225 | 5' | -55.3 | NC_003309.1 | + | 11589 | 0.76 | 0.215616 |
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Target: 5'- gUUCGcGUccgacaGCAGCCGCaCGCCGUCGGcaGCg -3' miRNA: 3'- gAAGC-CA------UGUCGGUG-GCGGCAGCU--UG- -5' |
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| 12225 | 5' | -55.3 | NC_003309.1 | + | 22400 | 0.77 | 0.169348 |
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Target: 5'- gCUUCGGcau-GCCGCUGCgCGUCGAACg -3' miRNA: 3'- -GAAGCCauguCGGUGGCG-GCAGCUUG- -5' |
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| 12225 | 5' | -55.3 | NC_003309.1 | + | 15597 | 0.79 | 0.14364 |
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Target: 5'- gCUUCGGcaguUGCuGGCCACCGaaGUCGAGCg -3' miRNA: 3'- -GAAGCC----AUG-UCGGUGGCggCAGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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