Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12227 | 3' | -54.1 | NC_003309.1 | + | 18753 | 0.65 | 0.788695 |
Target: 5'- cCGGCGGCGAuagucggcgccuugUCGAGCgugaUCGucucGCCgGCu -3' miRNA: 3'- cGUCGUCGCU--------------AGCUCG----AGCu---UGGaCG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 8533 | 0.66 | 0.781699 |
Target: 5'- cCGGCGGCGGcaCGAGCUuCGGcgcACC-GCu -3' miRNA: 3'- cGUCGUCGCUa-GCUCGA-GCU---UGGaCG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 23455 | 0.66 | 0.761295 |
Target: 5'- uGCGGCcgaucguaaCGAUCGAGCaggucgcccaaUCGAGCC-GCu -3' miRNA: 3'- -CGUCGuc-------GCUAGCUCG-----------AGCUUGGaCG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 52425 | 0.66 | 0.749837 |
Target: 5'- -uGGCgGGCGAucacccuguUCGAGCUUGGcgcaaucAUCUGCg -3' miRNA: 3'- cgUCG-UCGCU---------AGCUCGAGCU-------UGGACG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 32430 | 0.67 | 0.718959 |
Target: 5'- aCAGCAGCcaagCGAGagUCGAaggaaugcACCUGCc -3' miRNA: 3'- cGUCGUCGcua-GCUCg-AGCU--------UGGACG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 4616 | 0.67 | 0.686242 |
Target: 5'- gGCAGCGauGCGAUUGAuuGCU---GCCUGUg -3' miRNA: 3'- -CGUCGU--CGCUAGCU--CGAgcuUGGACG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 10723 | 0.67 | 0.686242 |
Target: 5'- cGCgAGCgcgAGCGAagccUCGGcgcGCUCGAGcuCCUGCu -3' miRNA: 3'- -CG-UCG---UCGCU----AGCU---CGAGCUU--GGACG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 48600 | 0.67 | 0.686242 |
Target: 5'- aGCaAGCAGCGGUaugucgugGGGCgCGAGCCUuCg -3' miRNA: 3'- -CG-UCGUCGCUAg-------CUCGaGCUUGGAcG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 43971 | 0.68 | 0.679632 |
Target: 5'- uGCAGCGGuCGcgcacgcucucgggcAUCGGGUUCG-GCUUGUg -3' miRNA: 3'- -CGUCGUC-GC---------------UAGCUCGAGCuUGGACG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 9528 | 0.68 | 0.664147 |
Target: 5'- uGCGcGCcGCG-UCGAGCgcgCGcaccguGACCUGCa -3' miRNA: 3'- -CGU-CGuCGCuAGCUCGa--GC------UUGGACG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 22456 | 0.68 | 0.653047 |
Target: 5'- uGC-GCGGCGAUCGcacgcugccGGCgUCGAGCgCgGCg -3' miRNA: 3'- -CGuCGUCGCUAGC---------UCG-AGCUUG-GaCG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 34545 | 0.68 | 0.641928 |
Target: 5'- cGCgAGCAGCGuagCGAGCacccgUCGAugUggGCg -3' miRNA: 3'- -CG-UCGUCGCua-GCUCG-----AGCUugGa-CG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 36315 | 0.68 | 0.641928 |
Target: 5'- aGCAGCAGaUGAagGAgcuGCUCGcACCcGCa -3' miRNA: 3'- -CGUCGUC-GCUagCU---CGAGCuUGGaCG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 10471 | 0.68 | 0.6308 |
Target: 5'- cGCGcGCAuGCcg-CGAGUUCG-GCCUGCg -3' miRNA: 3'- -CGU-CGU-CGcuaGCUCGAGCuUGGACG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 21754 | 0.68 | 0.619673 |
Target: 5'- --cGCAGgCGugcagCGAGCUCG-AUCUGCu -3' miRNA: 3'- cguCGUC-GCua---GCUCGAGCuUGGACG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 40193 | 0.69 | 0.608557 |
Target: 5'- cGCAGCAGCGGaccUCGAagucaGCgcaaacgCGAGCCg-- -3' miRNA: 3'- -CGUCGUCGCU---AGCU-----CGa------GCUUGGacg -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 11763 | 0.69 | 0.597463 |
Target: 5'- gGCGcGCccGGCGAUC-AGCcccgccaugCGGACCUGCg -3' miRNA: 3'- -CGU-CG--UCGCUAGcUCGa--------GCUUGGACG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 12024 | 0.69 | 0.597463 |
Target: 5'- cGCAGUggacGCGAUCGu-CUCGucgaaAGCCUGCu -3' miRNA: 3'- -CGUCGu---CGCUAGCucGAGC-----UUGGACG- -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 38253 | 0.69 | 0.575372 |
Target: 5'- aGCAcCAGCGAuugcaaaaaUCGAGC-CGAccGCCUGa -3' miRNA: 3'- -CGUcGUCGCU---------AGCUCGaGCU--UGGACg -5' |
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12227 | 3' | -54.1 | NC_003309.1 | + | 37228 | 0.69 | 0.574272 |
Target: 5'- aCAGCAGCGcGUCGAGgaCGAgguaaaggcgaacGCC-GCg -3' miRNA: 3'- cGUCGUCGC-UAGCUCgaGCU-------------UGGaCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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