Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12227 | 5' | -54.9 | NC_003309.1 | + | 7662 | 0.66 | 0.690006 |
Target: 5'- -aGCAcACGau--CGAUCGCGCGCGc -3' miRNA: 3'- caCGUuUGCcuucGCUAGCGCGCGCa -5' |
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12227 | 5' | -54.9 | NC_003309.1 | + | 45896 | 0.67 | 0.679012 |
Target: 5'- -aGUuGACGGuucAGC--UCGCGCGCGUu -3' miRNA: 3'- caCGuUUGCCu--UCGcuAGCGCGCGCA- -5' |
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12227 | 5' | -54.9 | NC_003309.1 | + | 15916 | 0.67 | 0.655789 |
Target: 5'- -cGCAGcacucgcGCGGAuacucuGCGAgCGCGUGCGc -3' miRNA: 3'- caCGUU-------UGCCUu-----CGCUaGCGCGCGCa -5' |
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12227 | 5' | -54.9 | NC_003309.1 | + | 42660 | 0.67 | 0.623577 |
Target: 5'- -aGCGAACGGGccGGCGAUgGCGUccuGuCGUc -3' miRNA: 3'- caCGUUUGCCU--UCGCUAgCGCG---C-GCA- -5' |
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12227 | 5' | -54.9 | NC_003309.1 | + | 22598 | 0.68 | 0.5793 |
Target: 5'- cGUGCAGuguCGGAcAGCGA-CGCGCaGCc- -3' miRNA: 3'- -CACGUUu--GCCU-UCGCUaGCGCG-CGca -5' |
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12227 | 5' | -54.9 | NC_003309.1 | + | 17773 | 0.68 | 0.568319 |
Target: 5'- -cGCAcACGGAucagccAGcCGuuuuUCGCGCGCGg -3' miRNA: 3'- caCGUuUGCCU------UC-GCu---AGCGCGCGCa -5' |
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12227 | 5' | -54.9 | NC_003309.1 | + | 27349 | 0.69 | 0.557392 |
Target: 5'- -cGCccaAAACGGGAGCGG-CGCG-GCGa -3' miRNA: 3'- caCG---UUUGCCUUCGCUaGCGCgCGCa -5' |
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12227 | 5' | -54.9 | NC_003309.1 | + | 10723 | 0.69 | 0.546526 |
Target: 5'- -cGCGAGCGcGAGCGAagccUCGgCGCGCu- -3' miRNA: 3'- caCGUUUGCcUUCGCU----AGC-GCGCGca -5' |
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12227 | 5' | -54.9 | NC_003309.1 | + | 9714 | 0.69 | 0.514366 |
Target: 5'- -cGuCGAGCGGuuGCcguguGUCGCGCGCGa -3' miRNA: 3'- caC-GUUUGCCuuCGc----UAGCGCGCGCa -5' |
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12227 | 5' | -54.9 | NC_003309.1 | + | 4505 | 0.7 | 0.452591 |
Target: 5'- cGUGCucaaaccACGGAAguucGCGAUUGCGuCGCGa -3' miRNA: 3'- -CACGuu-----UGCCUU----CGCUAGCGC-GCGCa -5' |
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12227 | 5' | -54.9 | NC_003309.1 | + | 5787 | 0.72 | 0.385969 |
Target: 5'- -cGCAAGCcuucgaGGAAGCGGaaCGUGCGCGg -3' miRNA: 3'- caCGUUUG------CCUUCGCUa-GCGCGCGCa -5' |
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12227 | 5' | -54.9 | NC_003309.1 | + | 16771 | 0.73 | 0.334262 |
Target: 5'- -gGCGAACGuGAGGCcgccGUCGCGCGCc- -3' miRNA: 3'- caCGUUUGC-CUUCGc---UAGCGCGCGca -5' |
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12227 | 5' | -54.9 | NC_003309.1 | + | 10897 | 0.74 | 0.295185 |
Target: 5'- -cGCc-GCGGucGCGGUCGCcGCGCGUu -3' miRNA: 3'- caCGuuUGCCuuCGCUAGCG-CGCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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