Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12228 | 3' | -55 | NC_003309.1 | + | 26754 | 0.66 | 0.726361 |
Target: 5'- cGGCG-AAGcccgccugaccuUGAACGGCAucuaaGCGGCGc -3' miRNA: 3'- uCCGCgUUC------------ACUUGCUGUug---CGCCGC- -5' |
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12228 | 3' | -55 | NC_003309.1 | + | 46674 | 0.66 | 0.715664 |
Target: 5'- uAGGCGCucAGGUGAcCGGgcCGGCGCGuaauGCGc -3' miRNA: 3'- -UCCGCG--UUCACUuGCU--GUUGCGC----CGC- -5' |
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12228 | 3' | -55 | NC_003309.1 | + | 53317 | 0.66 | 0.704883 |
Target: 5'- cGGCGCAuccgccGGGCgGGCAACGacgGGCGg -3' miRNA: 3'- uCCGCGUuca---CUUG-CUGUUGCg--CCGC- -5' |
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12228 | 3' | -55 | NC_003309.1 | + | 31886 | 0.66 | 0.694032 |
Target: 5'- -uGCGgAcGUGuuCGACAACGCGGUc -3' miRNA: 3'- ucCGCgUuCACuuGCUGUUGCGCCGc -5' |
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12228 | 3' | -55 | NC_003309.1 | + | 10703 | 0.67 | 0.63909 |
Target: 5'- cGGCGCGcucGAGCuccuGCuGCGCGGCGg -3' miRNA: 3'- uCCGCGUucaCUUGc---UGuUGCGCCGC- -5' |
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12228 | 3' | -55 | NC_003309.1 | + | 4630 | 0.68 | 0.616994 |
Target: 5'- gAGGCGCGAacuu-CGGCAGCGauGCGa -3' miRNA: 3'- -UCCGCGUUcacuuGCUGUUGCgcCGC- -5' |
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12228 | 3' | -55 | NC_003309.1 | + | 48810 | 0.68 | 0.573036 |
Target: 5'- cAGGCuGC---UGAACGGCgAAUGCGGCu -3' miRNA: 3'- -UCCG-CGuucACUUGCUG-UUGCGCCGc -5' |
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12228 | 3' | -55 | NC_003309.1 | + | 37595 | 0.73 | 0.338924 |
Target: 5'- aAGGUGCAAcgcuacGGCGACGAcCGCGGCGu -3' miRNA: 3'- -UCCGCGUUcac---UUGCUGUU-GCGCCGC- -5' |
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12228 | 3' | -55 | NC_003309.1 | + | 41444 | 0.73 | 0.314922 |
Target: 5'- uGGCGCAAGggacucccaGAcCGGCAagaaGCGCGGCa -3' miRNA: 3'- uCCGCGUUCa--------CUuGCUGU----UGCGCCGc -5' |
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12228 | 3' | -55 | NC_003309.1 | + | 7879 | 0.74 | 0.27083 |
Target: 5'- cGGCGUcGGUGAACGuCGGCGCGuaGCa -3' miRNA: 3'- uCCGCGuUCACUUGCuGUUGCGC--CGc -5' |
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12228 | 3' | -55 | NC_003309.1 | + | 32960 | 0.76 | 0.225823 |
Target: 5'- gAGGCGCGAGaGcGCGACGAcccugagcugauCGCGGCa -3' miRNA: 3'- -UCCGCGUUCaCuUGCUGUU------------GCGCCGc -5' |
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12228 | 3' | -55 | NC_003309.1 | + | 5360 | 0.76 | 0.205245 |
Target: 5'- cAGGCGCGggauGGUGAuguugccguucgagaGCGGCAGCGUGcGCGc -3' miRNA: 3'- -UCCGCGU----UCACU---------------UGCUGUUGCGC-CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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