Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12228 | 5' | -56.7 | NC_003309.1 | + | 7201 | 0.66 | 0.673991 |
Target: 5'- uACCGcCGUGAuGUCGUAaaGCCuGCCg -3' miRNA: 3'- -UGGCaGCGCUuCAGCAUg-CGGcUGGa -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 22475 | 0.66 | 0.652437 |
Target: 5'- cGCCGaCGCGgcGgcaUCGUGCGCggCGAUCg -3' miRNA: 3'- -UGGCaGCGCuuC---AGCAUGCG--GCUGGa -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 34385 | 0.66 | 0.641628 |
Target: 5'- aGCCuuggagCGCGAGGUCaaggaacUGCGCCgGACCa -3' miRNA: 3'- -UGGca----GCGCUUCAGc------AUGCGG-CUGGa -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 19494 | 0.67 | 0.619994 |
Target: 5'- gUCGUCGCGAuucGG-CGUGCgGCUGuACCa -3' miRNA: 3'- uGGCAGCGCU---UCaGCAUG-CGGC-UGGa -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 8266 | 0.67 | 0.619994 |
Target: 5'- gUCGcUCGCGAAuGUCGUcGCGUCGAUUUc -3' miRNA: 3'- uGGC-AGCGCUU-CAGCA-UGCGGCUGGA- -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 16834 | 0.68 | 0.52723 |
Target: 5'- cUCGUCGCGccccaccggaugcucGAGcUCGUccucgccgagauuGCGCCGGCCg -3' miRNA: 3'- uGGCAGCGC---------------UUC-AGCA-------------UGCGGCUGGa -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 4044 | 0.68 | 0.524109 |
Target: 5'- cAUCGgcUCGCaGggGUucaUGUGCGCCGAUCg -3' miRNA: 3'- -UGGC--AGCG-CuuCA---GCAUGCGGCUGGa -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 12850 | 0.69 | 0.4923 |
Target: 5'- aACCG-CGCGAGGUCGgcgaucaUGCuGUCGAUCa -3' miRNA: 3'- -UGGCaGCGCUUCAGC-------AUG-CGGCUGGa -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 5555 | 0.7 | 0.44395 |
Target: 5'- cGCCGaUCGCGGcgguGagGU-CGCCGACCa -3' miRNA: 3'- -UGGC-AGCGCUu---CagCAuGCGGCUGGa -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 578 | 0.7 | 0.43063 |
Target: 5'- uGCCGagcagcuUCGCGAAaggguuGUCGgguguuuucggcuuUGCGCCGACCa -3' miRNA: 3'- -UGGC-------AGCGCUU------CAGC--------------AUGCGGCUGGa -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 45281 | 0.71 | 0.388574 |
Target: 5'- uGCCGguacaGCGGauuuGGUCGaAUGCCGGCCg -3' miRNA: 3'- -UGGCag---CGCU----UCAGCaUGCGGCUGGa -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 7497 | 0.71 | 0.346028 |
Target: 5'- uUCGUC-CGGAccCGUACGCCGGCCg -3' miRNA: 3'- uGGCAGcGCUUcaGCAUGCGGCUGGa -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 10591 | 0.72 | 0.306926 |
Target: 5'- uGCCGUCGCGAcaugCGcuUGCGCCGccGCCUc -3' miRNA: 3'- -UGGCAGCGCUuca-GC--AUGCGGC--UGGA- -5' |
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12228 | 5' | -56.7 | NC_003309.1 | + | 16203 | 0.76 | 0.179384 |
Target: 5'- gGCCGgCGCGAacucgcaccAGUCGUcgACGCCGAUCg -3' miRNA: 3'- -UGGCaGCGCU---------UCAGCA--UGCGGCUGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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