miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12229 3' -56 NC_003309.1 + 6278 0.69 0.494121
Target:  5'- uCGGCGUCGaCGAGUuugucCGCA--UGCUCGu -3'
miRNA:   3'- -GCCGCAGCaGCUUA-----GCGUccACGAGC- -5'
12229 3' -56 NC_003309.1 + 22367 0.66 0.686244
Target:  5'- gCGGCcugcUCGUCGGcaagccgauGUgCGCcgaucAGGUGCUCGa -3'
miRNA:   3'- -GCCGc---AGCAGCU---------UA-GCG-----UCCACGAGC- -5'
12229 3' -56 NC_003309.1 + 46496 0.67 0.650614
Target:  5'- uGGcCGUCGgCGGGaacugcUCGCgcauagcgcgguugAGGUGCUCGa -3'
miRNA:   3'- gCC-GCAGCaGCUU------AGCG--------------UCCACGAGC- -5'
12229 3' -56 NC_003309.1 + 46565 0.7 0.473972
Target:  5'- uGGCGUCGaUCGAcggAUCGCAauuccccUGCUCGu -3'
miRNA:   3'- gCCGCAGC-AGCU---UAGCGUcc-----ACGAGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.