miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12229 5' -56.3 NC_003309.1 + 17794 0.66 0.633531
Target:  5'- uGCGcGUUCGgCGUGAUaCGGCGcacacGGAUc -3'
miRNA:   3'- uCGC-CAAGCgGCACUA-GCUGCu----CCUG- -5'
12229 5' -56.3 NC_003309.1 + 22502 0.68 0.568471
Target:  5'- cGUGGUUCGU--UGGUCGACGGcGGCc -3'
miRNA:   3'- uCGCCAAGCGgcACUAGCUGCUcCUG- -5'
12229 5' -56.3 NC_003309.1 + 25761 0.69 0.474701
Target:  5'- cGCGGagCGCa-UGAUCGGCGAcGGAg -3'
miRNA:   3'- uCGCCaaGCGgcACUAGCUGCU-CCUg -5'
12229 5' -56.3 NC_003309.1 + 5546 0.74 0.232631
Target:  5'- cGGCGGUgaggUCGCCGaccacgaucUGAUCGACGuucGGAUu -3'
miRNA:   3'- -UCGCCA----AGCGGC---------ACUAGCUGCu--CCUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.