Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12230 | 3' | -55.1 | NC_003309.1 | + | 10946 | 0.66 | 0.71548 |
Target: 5'- cGAGCgcCGUCUGCGcCGUC--GCCGCg -3' miRNA: 3'- -CUCGaaGCGGAUGUuGUAGccCGGCG- -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 16431 | 0.66 | 0.71548 |
Target: 5'- cAGCacgUCGCCgaccUGCAacgucACGUCGcGGCCGa -3' miRNA: 3'- cUCGa--AGCGG----AUGU-----UGUAGC-CCGGCg -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 40514 | 0.66 | 0.71548 |
Target: 5'- aGGGUccUGCCgaugcggACGugGUCuGGCCGCg -3' miRNA: 3'- -CUCGaaGCGGa------UGUugUAGcCCGGCG- -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 32845 | 0.66 | 0.704652 |
Target: 5'- -uGCUUCGCgaGCug---CGGGCCGa -3' miRNA: 3'- cuCGAAGCGgaUGuuguaGCCCGGCg -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 41888 | 0.66 | 0.693752 |
Target: 5'- aGGCUgcccCGUCUcuccgACGAgAUCGGGCCa- -3' miRNA: 3'- cUCGAa---GCGGA-----UGUUgUAGCCCGGcg -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 30829 | 0.66 | 0.687183 |
Target: 5'- gGAGCUUcucaacgagaaucgaCGCCgucuggcgcGCAAUGUCGcGCCGCg -3' miRNA: 3'- -CUCGAA---------------GCGGa--------UGUUGUAGCcCGGCG- -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 27962 | 0.67 | 0.660735 |
Target: 5'- gGAGCUccgcaguugcaUCGCagaUGCGucGCggCGGGCaCGCg -3' miRNA: 3'- -CUCGA-----------AGCGg--AUGU--UGuaGCCCG-GCG- -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 10415 | 0.67 | 0.660735 |
Target: 5'- cAGCUUCGCCgcaGCAGCAgcUCGaucGCCcuGCg -3' miRNA: 3'- cUCGAAGCGGa--UGUUGU--AGCc--CGG--CG- -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 48828 | 0.67 | 0.627472 |
Target: 5'- cAGCgUCGCCguucGCGUCaGGCUGCu -3' miRNA: 3'- cUCGaAGCGGauguUGUAGcCCGGCG- -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 47407 | 0.68 | 0.6053 |
Target: 5'- cGAGCcacgCGUCgacggGCAACAgUCGGGCaGCu -3' miRNA: 3'- -CUCGaa--GCGGa----UGUUGU-AGCCCGgCG- -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 9315 | 0.68 | 0.572241 |
Target: 5'- --aCUUCGCCaUGC-GCAUCaGGGCgGCu -3' miRNA: 3'- cucGAAGCGG-AUGuUGUAG-CCCGgCG- -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 32041 | 0.69 | 0.518237 |
Target: 5'- -cGCUUCGCUgGCGGC-UCGG-CCGUg -3' miRNA: 3'- cuCGAAGCGGaUGUUGuAGCCcGGCG- -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 12939 | 0.7 | 0.436571 |
Target: 5'- cGAGCg-CGCCgACGGCAUCGGagacaCCGCc -3' miRNA: 3'- -CUCGaaGCGGaUGUUGUAGCCc----GGCG- -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 38830 | 0.71 | 0.406962 |
Target: 5'- cGAGCUuucgggauaauccUCGCCUAUGGCAUCacuaucGGCUGUg -3' miRNA: 3'- -CUCGA-------------AGCGGAUGUUGUAGc-----CCGGCG- -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 4715 | 0.73 | 0.329325 |
Target: 5'- uGGCUacaUCGucaCCUGCAuguuCAUCGcGGCCGCg -3' miRNA: 3'- cUCGA---AGC---GGAUGUu---GUAGC-CCGGCG- -5' |
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12230 | 3' | -55.1 | NC_003309.1 | + | 13463 | 0.76 | 0.218493 |
Target: 5'- uGGGCUUUGCCacacGCAGCGUCGuaucGCCGCc -3' miRNA: 3'- -CUCGAAGCGGa---UGUUGUAGCc---CGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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