Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12230 | 5' | -58 | NC_003309.1 | + | 41690 | 0.66 | 0.544158 |
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Target: 5'- cCGGCcGCGCuccGCGuCGAGGUaGGUUCu -3' miRNA: 3'- -GCCGuCGUGu--UGCcGCUCCG-CCAAGu -5' |
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| 12230 | 5' | -58 | NC_003309.1 | + | 36778 | 0.66 | 0.544158 |
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Target: 5'- aGaGCGGaCGCu-CGGCGGuGGCGGUUgGg -3' miRNA: 3'- gC-CGUC-GUGuuGCCGCU-CCGCCAAgU- -5' |
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| 12230 | 5' | -58 | NC_003309.1 | + | 19383 | 0.66 | 0.544158 |
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Target: 5'- gCGGuCAGCgacacgaccgACGGCGGCGGaGUGGUUUu -3' miRNA: 3'- -GCC-GUCG----------UGUUGCCGCUcCGCCAAGu -5' |
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| 12230 | 5' | -58 | NC_003309.1 | + | 47492 | 0.66 | 0.533617 |
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Target: 5'- aGGCGGCGCAGCGacaccuucgccGCcGGGCGuGUcCAc -3' miRNA: 3'- gCCGUCGUGUUGC-----------CGcUCCGC-CAaGU- -5' |
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| 12230 | 5' | -58 | NC_003309.1 | + | 5059 | 0.67 | 0.48113 |
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Target: 5'- gCGGCGGCcggugccGCGACGGCGGucGGauuCGGgUCAa -3' miRNA: 3'- -GCCGUCG-------UGUUGCCGCU--CC---GCCaAGU- -5' |
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| 12230 | 5' | -58 | NC_003309.1 | + | 45227 | 0.67 | 0.462218 |
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Target: 5'- uCGGCGGCACuccAGCGcagaaUGGGGCGGUa-- -3' miRNA: 3'- -GCCGUCGUG---UUGCc----GCUCCGCCAagu -5' |
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| 12230 | 5' | -58 | NC_003309.1 | + | 26175 | 0.68 | 0.4466 |
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Target: 5'- gGGCGGCgGCAACGGCaaaccacccaugcuAGGCGGggaagCAg -3' miRNA: 3'- gCCGUCG-UGUUGCCGc-------------UCCGCCaa---GU- -5' |
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| 12230 | 5' | -58 | NC_003309.1 | + | 48809 | 0.7 | 0.344623 |
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Target: 5'- aGGCuGCugAACGGCGAauGCGGcuUUCGu -3' miRNA: 3'- gCCGuCGugUUGCCGCUc-CGCC--AAGU- -5' |
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| 12230 | 5' | -58 | NC_003309.1 | + | 37933 | 0.7 | 0.336514 |
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Target: 5'- uCGGauaAGCGCGGCcuuucGGCGGGGCGGc--- -3' miRNA: 3'- -GCCg--UCGUGUUG-----CCGCUCCGCCaagu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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