Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12233 | 3' | -56.4 | NC_003309.1 | + | 1176 | 0.66 | 0.654414 |
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Target: 5'- aUGAUcGGCUcaaauCGGCUGcCGUCGGcCGGCu -3' miRNA: 3'- -ACUA-CCGAc----GUCGAC-GCAGCU-GCUGc -5' |
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| 12233 | 3' | -56.4 | NC_003309.1 | + | 22605 | 0.66 | 0.654414 |
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Target: 5'- ---cGGCUGCcguGCaGUGUCGgacaGCGACGc -3' miRNA: 3'- acuaCCGACGu--CGaCGCAGC----UGCUGC- -5' |
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| 12233 | 3' | -56.4 | NC_003309.1 | + | 5063 | 0.67 | 0.599533 |
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Target: 5'- ---gGGCgcgGCGGCcgGUGccgCGACGGCGg -3' miRNA: 3'- acuaCCGa--CGUCGa-CGCa--GCUGCUGC- -5' |
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| 12233 | 3' | -56.4 | NC_003309.1 | + | 33802 | 0.68 | 0.53466 |
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Target: 5'- uUGuUGGCUGgcggguCAGCU-CGUCGAUGACc -3' miRNA: 3'- -ACuACCGAC------GUCGAcGCAGCUGCUGc -5' |
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| 12233 | 3' | -56.4 | NC_003309.1 | + | 24131 | 0.68 | 0.492798 |
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Target: 5'- uUGAUGcaacGCAGUUcCGUCGACGACGc -3' miRNA: 3'- -ACUACcga-CGUCGAcGCAGCUGCUGC- -5' |
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| 12233 | 3' | -56.4 | NC_003309.1 | + | 6844 | 0.68 | 0.489717 |
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Target: 5'- cGAUGGUcauaggcccaUGCGGCUGCGaguagcauccacgaUCGcacCGACGg -3' miRNA: 3'- aCUACCG----------ACGUCGACGC--------------AGCu--GCUGC- -5' |
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| 12233 | 3' | -56.4 | NC_003309.1 | + | 32794 | 0.7 | 0.377725 |
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Target: 5'- aGAUGGCUGCuGaUGCGUUGGacaGGCu -3' miRNA: 3'- aCUACCGACGuCgACGCAGCUg--CUGc -5' |
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| 12233 | 3' | -56.4 | NC_003309.1 | + | 15324 | 0.71 | 0.360371 |
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Target: 5'- cGAUGGCUGCuGC-GCGUCgGAUGuCu -3' miRNA: 3'- aCUACCGACGuCGaCGCAG-CUGCuGc -5' |
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| 12233 | 3' | -56.4 | NC_003309.1 | + | 37238 | 0.72 | 0.319467 |
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Target: 5'- aGgcGGCgcuacaGCAGC-GCGUCGAgGACGa -3' miRNA: 3'- aCuaCCGa-----CGUCGaCGCAGCUgCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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