Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12234 | 3' | -54.7 | NC_003309.1 | + | 19072 | 0.66 | 0.784547 |
Target: 5'- cGGCGGUGGCUGUaCCGaaggCGGAa- -3' miRNA: 3'- cCCGCUACUGACAaGGUgag-GCCUgg -5' |
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12234 | 3' | -54.7 | NC_003309.1 | + | 10665 | 0.66 | 0.774649 |
Target: 5'- cGGGCGAUGAUcg--CCGCguaCGGcauGCCc -3' miRNA: 3'- -CCCGCUACUGacaaGGUGag-GCC---UGG- -5' |
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12234 | 3' | -54.7 | NC_003309.1 | + | 14869 | 0.66 | 0.772651 |
Target: 5'- -uGCGAUGACgccagccugCCGCcccgcuUCCGGACUg -3' miRNA: 3'- ccCGCUACUGacaa-----GGUG------AGGCCUGG- -5' |
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12234 | 3' | -54.7 | NC_003309.1 | + | 15327 | 0.7 | 0.507963 |
Target: 5'- cGGCGAUGGCUGcUgCGCgUCGGAUg -3' miRNA: 3'- cCCGCUACUGACaAgGUGaGGCCUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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