Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12235 | 5' | -57.4 | NC_003309.1 | + | 15019 | 0.66 | 0.604723 |
Target: 5'- aCGgAAGGUca-GCgACGGccGCCCCu -3' miRNA: 3'- aGCgUUCCGaagCGgUGCCaaCGGGG- -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 6371 | 0.66 | 0.584159 |
Target: 5'- cCGCGuuacccCUUCGCCGCGGUgggcuuggcgcuuucGCCCUu -3' miRNA: 3'- aGCGUucc---GAAGCGGUGCCAa--------------CGGGG- -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 32388 | 0.66 | 0.583081 |
Target: 5'- -gGCGAcGGC-UCGCUGCGGcggccgUGUCCUg -3' miRNA: 3'- agCGUU-CCGaAGCGGUGCCa-----ACGGGG- -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 22627 | 0.67 | 0.540336 |
Target: 5'- gCGCcGGGCacgccgUCGCCAcCGGcUGCCg- -3' miRNA: 3'- aGCGuUCCGa-----AGCGGU-GCCaACGGgg -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 668 | 0.67 | 0.540336 |
Target: 5'- aCGCAGGGCgcUUGCCACGaacgacGUCUCg -3' miRNA: 3'- aGCGUUCCGa-AGCGGUGCcaa---CGGGG- -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 10669 | 0.67 | 0.508981 |
Target: 5'- cUCGC-GGGCgaugaUCGCCGCGuacggcaUGCCCg -3' miRNA: 3'- -AGCGuUCCGa----AGCGGUGCca-----ACGGGg -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 523 | 0.67 | 0.508981 |
Target: 5'- gCGCugccGGC-UCGCCG-GGUcgaGCCCCa -3' miRNA: 3'- aGCGuu--CCGaAGCGGUgCCAa--CGGGG- -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 26492 | 0.67 | 0.497674 |
Target: 5'- aCGCAgAGGCUUgGCUcgcgcgaACGGcUGCgCCg -3' miRNA: 3'- aGCGU-UCCGAAgCGG-------UGCCaACGgGG- -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 48919 | 0.68 | 0.488507 |
Target: 5'- -aGCAcGGU-UCGCCGCGGcacGCCgCCa -3' miRNA: 3'- agCGUuCCGaAGCGGUGCCaa-CGG-GG- -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 11503 | 0.68 | 0.448783 |
Target: 5'- aCGCGAGGC-UCGCCGugucgaucgcgcCGGcgGCUCg -3' miRNA: 3'- aGCGUUCCGaAGCGGU------------GCCaaCGGGg -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 853 | 0.68 | 0.439133 |
Target: 5'- cUGcCAAGGCUccagCGUUACcaGUUGCCCCu -3' miRNA: 3'- aGC-GUUCCGAa---GCGGUGc-CAACGGGG- -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 10491 | 0.69 | 0.420193 |
Target: 5'- aUCGCGAGGCuUUCGCgcaucgCGCGcaUGCCgCg -3' miRNA: 3'- -AGCGUUCCG-AAGCG------GUGCcaACGGgG- -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 43881 | 0.69 | 0.41091 |
Target: 5'- aCGCAAGa---CGCCACGGcUGgCCCa -3' miRNA: 3'- aGCGUUCcgaaGCGGUGCCaACgGGG- -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 51699 | 0.71 | 0.310018 |
Target: 5'- gUGCGAGGCgugauguagCGCCcCGGauUUGCCCa -3' miRNA: 3'- aGCGUUCCGaa-------GCGGuGCC--AACGGGg -5' |
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12235 | 5' | -57.4 | NC_003309.1 | + | 1215 | 0.73 | 0.229126 |
Target: 5'- aUCGCAcacgacGGCgacgcgccgUCGCCugGGUUGCCg- -3' miRNA: 3'- -AGCGUu-----CCGa--------AGCGGugCCAACGGgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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