Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12236 | 3' | -52.9 | NC_003309.1 | + | 33656 | 0.66 | 0.843677 |
Target: 5'- -----aGCGAACGGCuGGCugaGGCCGGc -3' miRNA: 3'- gcaaaaCGUUUGUCGcCUG---CCGGCCu -5' |
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12236 | 3' | -52.9 | NC_003309.1 | + | 41289 | 0.66 | 0.834729 |
Target: 5'- ----aUGgGAACGGCuugacgcuGGCGGCCGGGc -3' miRNA: 3'- gcaaaACgUUUGUCGc-------CUGCCGGCCU- -5' |
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12236 | 3' | -52.9 | NC_003309.1 | + | 34533 | 0.66 | 0.816183 |
Target: 5'- -----aGCGAGCAcccgucgauGUGGGCGGCCGu- -3' miRNA: 3'- gcaaaaCGUUUGU---------CGCCUGCCGGCcu -5' |
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12236 | 3' | -52.9 | NC_003309.1 | + | 32925 | 0.67 | 0.796836 |
Target: 5'- -----gGCAAGCAacaaCGGcuauGCGGCCGGAc -3' miRNA: 3'- gcaaaaCGUUUGUc---GCC----UGCCGGCCU- -5' |
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12236 | 3' | -52.9 | NC_003309.1 | + | 26395 | 0.69 | 0.635559 |
Target: 5'- aGUcg-GCAAccGCcgcGCGGACGGCgGGAu -3' miRNA: 3'- gCAaaaCGUU--UGu--CGCCUGCCGgCCU- -5' |
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12236 | 3' | -52.9 | NC_003309.1 | + | 34836 | 0.72 | 0.482661 |
Target: 5'- -----gGCAAAC-GUGGAgGGCCGGGa -3' miRNA: 3'- gcaaaaCGUUUGuCGCCUgCCGGCCU- -5' |
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12236 | 3' | -52.9 | NC_003309.1 | + | 5077 | 0.72 | 0.472334 |
Target: 5'- gGUUgUGCAuGCacgGGCGcGGCGGCCGGu -3' miRNA: 3'- gCAAaACGUuUG---UCGC-CUGCCGGCCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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