Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12236 | 5' | -58.9 | NC_003309.1 | + | 22441 | 0.66 | 0.552453 |
Target: 5'- cGCU--GCCGGCGUCGagcGCGGC-GCGCg -3' miRNA: 3'- cCGGucUGGCUGCGGC---UGCCGaUGUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 20772 | 0.66 | 0.551406 |
Target: 5'- aGCCAGcCCGGCaCCGGCGuucgauuGCUGCu- -3' miRNA: 3'- cCGGUCuGGCUGcGGCUGC-------CGAUGug -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 45256 | 0.66 | 0.549314 |
Target: 5'- uGCCGGccGCCGcgugcgccucgaauACGUCGGCGGCacuccaGCGCa -3' miRNA: 3'- cCGGUC--UGGC--------------UGCGGCUGCCGa-----UGUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 7749 | 0.66 | 0.531637 |
Target: 5'- cGCCGGugCGACGCCGAgauacccCUucGCACc -3' miRNA: 3'- cCGGUCugGCUGCGGCUgcc----GA--UGUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 32745 | 0.66 | 0.531637 |
Target: 5'- aGGCguGcgcGCUGuCGCCuACGaGCUGCGCa -3' miRNA: 3'- -CCGguC---UGGCuGCGGcUGC-CGAUGUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 13979 | 0.66 | 0.521335 |
Target: 5'- cGGCCGcACCGAcgaCGCgGGCGGgaGCcCg -3' miRNA: 3'- -CCGGUcUGGCU---GCGgCUGCCgaUGuG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 26395 | 0.66 | 0.521335 |
Target: 5'- aGUCGGcaACCGccGCGCgGACGGCgggaucgaaUGCACg -3' miRNA: 3'- cCGGUC--UGGC--UGCGgCUGCCG---------AUGUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 49338 | 0.66 | 0.500973 |
Target: 5'- cGGCCGcGACCaacuGGCGCUuaACGGUUGCGa -3' miRNA: 3'- -CCGGU-CUGG----CUGCGGc-UGCCGAUGUg -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 18778 | 0.66 | 0.498956 |
Target: 5'- cGGCaAGAUCGccgugaaacgauCGCCGGCGGCgauagucgGCGCc -3' miRNA: 3'- -CCGgUCUGGCu-----------GCGGCUGCCGa-------UGUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 4277 | 0.67 | 0.490924 |
Target: 5'- cGGCU-GACgaucaccuuCGACGCUG-CGGCUGCGa -3' miRNA: 3'- -CCGGuCUG---------GCUGCGGCuGCCGAUGUg -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 18726 | 0.67 | 0.490924 |
Target: 5'- aGCguGAUCGucuCGCCGGCuGCgGCGCg -3' miRNA: 3'- cCGguCUGGCu--GCGGCUGcCGaUGUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 35498 | 0.67 | 0.480969 |
Target: 5'- -aCCAacucGCCGACGCCGAUuGCaGCGCa -3' miRNA: 3'- ccGGUc---UGGCUGCGGCUGcCGaUGUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 44635 | 0.67 | 0.479979 |
Target: 5'- cGCCGGACagagaaaCGACGUgGAgGGUUGgGCg -3' miRNA: 3'- cCGGUCUG-------GCUGCGgCUgCCGAUgUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 22659 | 0.67 | 0.475043 |
Target: 5'- cGGCaccaucGCCcACGCCGGCGGCgcuggaagcgccggGCACg -3' miRNA: 3'- -CCGguc---UGGcUGCGGCUGCCGa-------------UGUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 35036 | 0.67 | 0.461361 |
Target: 5'- cGCCuGugCGACGCCGACGacGUgaucgAUAUg -3' miRNA: 3'- cCGGuCugGCUGCGGCUGC--CGa----UGUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 4877 | 0.67 | 0.442183 |
Target: 5'- uGCCAGcgcuugcacccGCUGAU--UGACGGCUGCGCg -3' miRNA: 3'- cCGGUC-----------UGGCUGcgGCUGCCGAUGUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 44881 | 0.67 | 0.442183 |
Target: 5'- uGCCGuacacGGCUGAcucagguuCGCCGGCGGCgauccGCGCa -3' miRNA: 3'- cCGGU-----CUGGCU--------GCGGCUGCCGa----UGUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 30999 | 0.68 | 0.432765 |
Target: 5'- cGCCAGAUCG-CGa--GCGGCUACAa -3' miRNA: 3'- cCGGUCUGGCuGCggcUGCCGAUGUg -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 3579 | 0.68 | 0.432765 |
Target: 5'- aGCCAGccccACUGGCGCCcgaccgcguaGGCGGCguauCGCg -3' miRNA: 3'- cCGGUC----UGGCUGCGG----------CUGCCGau--GUG- -5' |
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12236 | 5' | -58.9 | NC_003309.1 | + | 16083 | 0.68 | 0.432765 |
Target: 5'- cGGCUguGCCGACGCCccCGGaugcgaaUGCACg -3' miRNA: 3'- -CCGGucUGGCUGCGGcuGCCg------AUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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