Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12238 | 5' | -55.8 | NC_003309.1 | + | 18728 | 0.66 | 0.628851 |
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Target: 5'- -cGAGCGUGa--UCG-UCUCGCCGg- -3' miRNA: 3'- aaCUCGCACguaAGCuAGGGCGGCag -5' |
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| 12238 | 5' | -55.8 | NC_003309.1 | + | 18498 | 0.67 | 0.606596 |
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Target: 5'- aUGAcGCc-GUAUcgcgCGAUCCCGUCGUCg -3' miRNA: 3'- aACU-CGcaCGUAa---GCUAGGGCGGCAG- -5' |
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| 12238 | 5' | -55.8 | NC_003309.1 | + | 2228 | 0.67 | 0.584432 |
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Target: 5'- aUGAGCGcaacagcGCcgUCGAUUuuGuuGUCg -3' miRNA: 3'- aACUCGCa------CGuaAGCUAGggCggCAG- -5' |
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| 12238 | 5' | -55.8 | NC_003309.1 | + | 13239 | 0.67 | 0.562429 |
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Target: 5'- cUG-GCGcGCugaccgaGAUCCCGCCGUUg -3' miRNA: 3'- aACuCGCaCGuaag---CUAGGGCGGCAG- -5' |
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| 12238 | 5' | -55.8 | NC_003309.1 | + | 15497 | 0.68 | 0.50856 |
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Target: 5'- cUGcGGCG-GCAUuucuucguUCGggUCCGCCGUCg -3' miRNA: 3'- aAC-UCGCaCGUA--------AGCuaGGGCGGCAG- -5' |
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| 12238 | 5' | -55.8 | NC_003309.1 | + | 18970 | 0.69 | 0.477294 |
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Target: 5'- gUUGAuCGUGUAgaCGAUCUgGCCGUUg -3' miRNA: 3'- -AACUcGCACGUaaGCUAGGgCGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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