Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12239 | 3' | -57.1 | NC_003309.1 | + | 6370 | 0.66 | 0.62589 |
Target: 5'- cGCGuuaCCcCUUCGCCGCGgUgGGCUUg- -3' miRNA: 3'- -UGC---GGaGAAGCGGCGCgAgCUGAAgu -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 37296 | 0.66 | 0.62589 |
Target: 5'- gACGCC-CUgaaucUCGCCgGCGCgUCGAUggCGg -3' miRNA: 3'- -UGCGGaGA-----AGCGG-CGCG-AGCUGaaGU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 3459 | 0.66 | 0.614971 |
Target: 5'- cGCGCgUCUUCGCCugcucaugcCGCUUGACc--- -3' miRNA: 3'- -UGCGgAGAAGCGGc--------GCGAGCUGaagu -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 8918 | 0.66 | 0.582337 |
Target: 5'- aACGCCUCcgCGaCCGCGgUCG-CaUCGu -3' miRNA: 3'- -UGCGGAGaaGC-GGCGCgAGCuGaAGU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 44186 | 0.66 | 0.571529 |
Target: 5'- gACGCCcgCggcgCGCUGcCGCUCGGCg--- -3' miRNA: 3'- -UGCGGa-Gaa--GCGGC-GCGAGCUGaagu -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 10578 | 0.67 | 0.550067 |
Target: 5'- uGCGCUUgCgcCGCCGC-CUCGACggCAc -3' miRNA: 3'- -UGCGGA-GaaGCGGCGcGAGCUGaaGU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 46247 | 0.67 | 0.539429 |
Target: 5'- aACGCCUgaacCUUCGCgCGuUGCUCGucgggcgacaGCUUCGc -3' miRNA: 3'- -UGCGGA----GAAGCG-GC-GCGAGC----------UGAAGU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 12606 | 0.67 | 0.528861 |
Target: 5'- cCGCCUCggcCGUguugaUGCGCUCGAUcUCGg -3' miRNA: 3'- uGCGGAGaa-GCG-----GCGCGAGCUGaAGU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 11226 | 0.67 | 0.528861 |
Target: 5'- uGCGCgUCgUCuGCCGCGCgCGACg--- -3' miRNA: 3'- -UGCGgAGaAG-CGGCGCGaGCUGaagu -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 10372 | 0.67 | 0.528861 |
Target: 5'- aGCGCCgccugCGCCGC-CUCGugUUg- -3' miRNA: 3'- -UGCGGagaa-GCGGCGcGAGCugAAgu -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 7347 | 0.67 | 0.528861 |
Target: 5'- cGCGCCUaagcgcCUUCGCCGa-CUgCGAUUUCGc -3' miRNA: 3'- -UGCGGA------GAAGCGGCgcGA-GCUGAAGU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 6335 | 0.68 | 0.497646 |
Target: 5'- uCGCC-CUUCGCCGaCGCuuUCGcaccCUUCAc -3' miRNA: 3'- uGCGGaGAAGCGGC-GCG--AGCu---GAAGU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 9321 | 0.68 | 0.487423 |
Target: 5'- uCGCCUaCUUCGCCauGCGCaucagggCGGCUUgAa -3' miRNA: 3'- uGCGGA-GAAGCGG--CGCGa------GCUGAAgU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 47607 | 0.69 | 0.437904 |
Target: 5'- cUGCCcggUCgUCGCCGCGCcgucCGGCUUUAc -3' miRNA: 3'- uGCGG---AGaAGCGGCGCGa---GCUGAAGU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 42775 | 0.69 | 0.409613 |
Target: 5'- gGCGCUUCUugcagUCGuuGaCGCUCuACUUCGg -3' miRNA: 3'- -UGCGGAGA-----AGCggC-GCGAGcUGAAGU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 5574 | 0.7 | 0.356585 |
Target: 5'- aACGCCuugUCUUCGCgCGCGC-CGAUcgCGg -3' miRNA: 3'- -UGCGG---AGAAGCG-GCGCGaGCUGaaGU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 14153 | 0.71 | 0.328751 |
Target: 5'- gACGaCCUCgucccacaucaucUUGCCGCGCUCGGCg--- -3' miRNA: 3'- -UGC-GGAGa------------AGCGGCGCGAGCUGaagu -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 5201 | 0.71 | 0.323223 |
Target: 5'- cGCGaCUUCUUCgccgaaGCCGCGCUCGAUcgccaguUUCGa -3' miRNA: 3'- -UGC-GGAGAAG------CGGCGCGAGCUG-------AAGU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 10935 | 0.74 | 0.216099 |
Target: 5'- uGCGCCg--UCGCCGCGCUuucagucgcCGuCUUCAg -3' miRNA: 3'- -UGCGGagaAGCGGCGCGA---------GCuGAAGU- -5' |
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12239 | 3' | -57.1 | NC_003309.1 | + | 53256 | 0.74 | 0.194427 |
Target: 5'- cGCGCCgUCUUCGCCGCGU--GACagUCAc -3' miRNA: 3'- -UGCGG-AGAAGCGGCGCGagCUGa-AGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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