Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1224 | 3' | -49.1 | NC_001266.1 | + | 26211 | 0.66 | 0.998726 |
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Target: 5'- cGCAUG-GAaauuaGACACGGGuuCGGAcaACGCg -3' miRNA: 3'- cUGUACuCUg----UUGUGUCU--GCCU--UGCG- -5' |
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| 1224 | 3' | -49.1 | NC_001266.1 | + | 95122 | 0.67 | 0.997356 |
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Target: 5'- cAUAUGGGuagcCAACGCAGACGauACGUc -3' miRNA: 3'- cUGUACUCu---GUUGUGUCUGCcuUGCG- -5' |
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| 1224 | 3' | -49.1 | NC_001266.1 | + | 59202 | 0.67 | 0.996868 |
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Target: 5'- aGACAacgaAGAgGACACGGACGGuguAUGUu -3' miRNA: 3'- -CUGUac--UCUgUUGUGUCUGCCu--UGCG- -5' |
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| 1224 | 3' | -49.1 | NC_001266.1 | + | 83616 | 0.67 | 0.996307 |
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Target: 5'- -cCGUG-GACGACGaaucuGGAUGGggUGCg -3' miRNA: 3'- cuGUACuCUGUUGUg----UCUGCCuuGCG- -5' |
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| 1224 | 3' | -49.1 | NC_001266.1 | + | 13625 | 0.69 | 0.989668 |
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Target: 5'- aACAUuGGACGugACAGuucGCGG-ACGCa -3' miRNA: 3'- cUGUAcUCUGUugUGUC---UGCCuUGCG- -5' |
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| 1224 | 3' | -49.1 | NC_001266.1 | + | 5903 | 0.69 | 0.988226 |
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Target: 5'- cGCGUcGAGu--ACACcguaucgaAGACGGAGCGCg -3' miRNA: 3'- cUGUA-CUCuguUGUG--------UCUGCCUUGCG- -5' |
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| 1224 | 3' | -49.1 | NC_001266.1 | + | 153955 | 0.69 | 0.988226 |
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Target: 5'- cGCGUcGAGu--ACACcguaucgaAGACGGAGCGCg -3' miRNA: 3'- cUGUA-CUCuguUGUG--------UCUGCCUUGCG- -5' |
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| 1224 | 3' | -49.1 | NC_001266.1 | + | 153092 | 1.12 | 0.0092 |
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Target: 5'- cGACAUGAGACAACACAGACGGAACGCc -3' miRNA: 3'- -CUGUACUCUGUUGUGUCUGCCUUGCG- -5' |
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| 1224 | 3' | -49.1 | NC_001266.1 | + | 6766 | 1.12 | 0.0092 |
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Target: 5'- cGACAUGAGACAACACAGACGGAACGCc -3' miRNA: 3'- -CUGUACUCUGUUGUGUCUGCCUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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