Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1224 | 5' | -49.3 | NC_001266.1 | + | 127763 | 0.66 | 0.998295 |
Target: 5'- gAGUaugugGUAAAUGGAcGUcgCUGCGUCGa -3' miRNA: 3'- gUCG-----CAUUUACCUaCGuaGAUGCGGU- -5' |
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1224 | 5' | -49.3 | NC_001266.1 | + | 6546 | 0.68 | 0.990086 |
Target: 5'- uGGCGUAGAUGcAUcCAUUUACGCUg -3' miRNA: 3'- gUCGCAUUUACcUAcGUAGAUGCGGu -5' |
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1224 | 5' | -49.3 | NC_001266.1 | + | 153312 | 0.68 | 0.990086 |
Target: 5'- uGGCGUAGAUGcAUcCAUUUACGCUg -3' miRNA: 3'- gUCGCAUUUACcUAcGUAGAUGCGGu -5' |
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1224 | 5' | -49.3 | NC_001266.1 | + | 105238 | 0.69 | 0.981379 |
Target: 5'- aCAGCGUcuuucuucAAGUGGGUGUAUUuuaggUACGCa- -3' miRNA: 3'- -GUCGCA--------UUUACCUACGUAG-----AUGCGgu -5' |
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1224 | 5' | -49.3 | NC_001266.1 | + | 148112 | 0.71 | 0.944051 |
Target: 5'- -uGuCGUAAAUGGAUaUAUCUACGUCGu -3' miRNA: 3'- guC-GCAUUUACCUAcGUAGAUGCGGU- -5' |
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1224 | 5' | -49.3 | NC_001266.1 | + | 11746 | 0.71 | 0.944051 |
Target: 5'- -uGuCGUAAAUGGAUaUAUCUACGUCGu -3' miRNA: 3'- guC-GCAUUUACCUAcGUAGAUGCGGU- -5' |
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1224 | 5' | -49.3 | NC_001266.1 | + | 153127 | 1.09 | 0.011778 |
Target: 5'- gCAGCGUAAAUGGAUGCAUCUACGCCAu -3' miRNA: 3'- -GUCGCAUUUACCUACGUAGAUGCGGU- -5' |
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1224 | 5' | -49.3 | NC_001266.1 | + | 6731 | 1.09 | 0.011778 |
Target: 5'- gCAGCGUAAAUGGAUGCAUCUACGCCAu -3' miRNA: 3'- -GUCGCAUUUACCUACGUAGAUGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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