Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12241 | 3' | -55.6 | NC_003309.1 | + | 50252 | 0.69 | 0.492534 |
Target: 5'- -gCGAAaGCUGAucGUCGCgcgCGCGUGAu -3' miRNA: 3'- caGCUUaCGACUu-CGGCGa--GCGCGCU- -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 48713 | 0.7 | 0.451944 |
Target: 5'- gGUCGAcUGCcaauGCCGCgUGCGCGAu -3' miRNA: 3'- -CAGCUuACGacuuCGGCGaGCGCGCU- -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 46982 | 0.66 | 0.687337 |
Target: 5'- uGUCGAcgcucggauagauGUGCgu--GCCGUcgUCGUGCGAa -3' miRNA: 3'- -CAGCU-------------UACGacuuCGGCG--AGCGCGCU- -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 45263 | 0.73 | 0.310314 |
Target: 5'- gGUCGAAUGCc--GGCCGC-CGCGUGc -3' miRNA: 3'- -CAGCUUACGacuUCGGCGaGCGCGCu -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 45036 | 0.69 | 0.48943 |
Target: 5'- cUCGGucuucagguacugcGUGCaGAAcuGCCGCUUGCGUGAu -3' miRNA: 3'- cAGCU--------------UACGaCUU--CGGCGAGCGCGCU- -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 44949 | 0.67 | 0.611157 |
Target: 5'- gGUCGGAUGU---AGCacuucaagCGCUCGUGCGGc -3' miRNA: 3'- -CAGCUUACGacuUCG--------GCGAGCGCGCU- -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 31971 | 0.67 | 0.611157 |
Target: 5'- ------gGCgcAAGCCGCUCGCGUGGa -3' miRNA: 3'- cagcuuaCGacUUCGGCGAGCGCGCU- -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 26503 | 0.68 | 0.55521 |
Target: 5'- -cUGGcUGCUgcacgcaGAGGCUugGCUCGCGCGAa -3' miRNA: 3'- caGCUuACGA-------CUUCGG--CGAGCGCGCU- -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 23595 | 0.66 | 0.666463 |
Target: 5'- -cCGGAUggucGUUGAGGCUGacgaUCGCGCGc -3' miRNA: 3'- caGCUUA----CGACUUCGGCg---AGCGCGCu -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 18434 | 0.66 | 0.677469 |
Target: 5'- cGUCGGAUcGUUGuAGCUGacgaGCGCGAc -3' miRNA: 3'- -CAGCUUA-CGACuUCGGCgag-CGCGCU- -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 16781 | 0.7 | 0.413251 |
Target: 5'- cGUUGAAcacgGCgaacgUGAGGCCGCcgUCGCGCGc -3' miRNA: 3'- -CAGCUUa---CG-----ACUUCGGCG--AGCGCGCu -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 12649 | 0.69 | 0.482223 |
Target: 5'- -gCGAGccgcUGCUcGAgauAGCCGCgcucgCGCGCGAc -3' miRNA: 3'- caGCUU----ACGA-CU---UCGGCGa----GCGCGCU- -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 12514 | 0.67 | 0.578113 |
Target: 5'- cGUCGAcgGC-GAAacucGUCGCUCaCGCGAu -3' miRNA: 3'- -CAGCUuaCGaCUU----CGGCGAGcGCGCU- -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 12004 | 0.69 | 0.459918 |
Target: 5'- cGUCGAAagccUGCUGcccgcgcgcggcAGCCGCggCGCGCGc -3' miRNA: 3'- -CAGCUU----ACGACu-----------UCGGCGa-GCGCGCu -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 10696 | 0.66 | 0.644365 |
Target: 5'- cUCGAGcuccUGCUGcgcggcGGCCGuCUCGCggGCGAu -3' miRNA: 3'- cAGCUU----ACGACu-----UCGGC-GAGCG--CGCU- -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 9714 | 0.67 | 0.633293 |
Target: 5'- cGUCGA--GCgGuuGCCGUgugucgCGCGCGAu -3' miRNA: 3'- -CAGCUuaCGaCuuCGGCGa-----GCGCGCU- -5' |
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12241 | 3' | -55.6 | NC_003309.1 | + | 506 | 0.66 | 0.644365 |
Target: 5'- gGUCGAGccccagcauUGCgccGAAGCCGCcauUUGCGCc- -3' miRNA: 3'- -CAGCUU---------ACGa--CUUCGGCG---AGCGCGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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